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Understanding seasonal weight loss tolerance in dairy goats: a transcriptomics approach
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Orientador(es)
Resumo(s)
Background: Seasonal weight loss (SWL) is a very important limitation to the production of ruminants in the
Mediterranean and Tropical regions. In these areas, long dry seasons lead to poor pastures with low nutritional
value. During the dry season, ruminants, particularly those raised in extensive production systems, lose around 30%
of their body weight. Seasonal weight loss has important consequences on animal productive performance and
health. In this study, RNA sequencing was used to characterize feed restriction effects in dairy goat of 2 breeds with
different SWL tolerance: Majorera (tolerant) and Palmera (susceptible). Nine Majorera and ten Palmera goats were
randomly distributed in a control and a restricted group: Majorera Control (adequately fed; MC; n = 4), Palmera
Control (adequately fed; PC; n = 6), Majorera Restricted (feed restricted; ME; n = 5) and Palmera Restricted (feed
restricted; PE; n = 4). On day 22 of the trial, mammary gland biopsies were collected for transcriptomics analysis.
Results: From these samples, 24,260 unique transcripts were identified. From those, 82 transcripts were differentially
expressed between MC and ME, 99 between PC and PE, twelve between both control groups and twenty-nine
between both restricted groups.
Conclusions: Feed restriction affected several biochemical pathways in both breeds such as: carbohydrate and lipid
transport; intracellular trafficking, RNA processing and signal transduction. This research also highlights the
importance or involvement of the genes in tolerance (ENPP1, S-LZ, MT2A and GPNB) and susceptibility (GPD1, CTPS1,
ELOVL6 and NR4A1) to SWL with respectively higher expression in the Majorera restriced group and the Palmera
restricted group in comparison to the control groups. In addition, results from the study may be extrapolated to
other dairy ruminant species
Descrição
Research Article
Palavras-chave
goat mammary gland transcriptomics RNA-sequencing seasonal weight loss
Contexto Educativo
Citação
Parreira et al. BMC Genomics (2020) 21:629
Editora
BMC