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Modulation of rye rDNA chromatin conformation induced by high temperature stress
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Orientador(es)
Resumo(s)
Epigenetic modulation has been correlated with expression patterns alterations involved in
plant response to stress. However, the influence of abiotic stress, namely high temperature, in
chromatin organization of plant nuclei is poorly known. The aim of this work was the analysis of
rDNA chromatin organization in interphase nuclei of rye root-tip and coleoptiles from high
temperature treated (HTS) and untreated plantlets, through Fluorescent In Situ Hybridization (FISH)
using rDNA probes. Rye seeds were germinated in controlled conditions and HTS treated plantlets
were subject to a period of 4 hours at 40oC. FISH was performed successfully using a new rDNA
probe produced by PCR that can replace with advantage the pTa71 probe and the results obtained with
both probes revealed significant differences in rDNA chromatin organization patterns between control
and HTS in interphase nuclei from both tissues analyzed. The detected rDNA chromatin
decondensation provoked by HTS may be involved in a greater potential for transcription necessary to
the amendment induced by HTS and emphasize the role of chromatin epigenetic remodelling in plant
adjustment pathways under stress conditions.
Descrição
Report - Bolsa de integração na Investigação
Palavras-chave
rye rDNA chromatin stress
Contexto Educativo
Citação
Brazão, J.M.H.S. - Modulation of rye rDNA chromatin conformation induced by high temperature stress. Lisboa: ISA/CBAA, 2010