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The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium

2020-05-16Journal article Open access
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dc.contributor.authorAmaral, Ana
dc.contributor.authorFernandes, Carina
dc.contributor.authorRebordão, Maria Rosa
dc.contributor.authorSzóstek-Mioduchowska, Anna
dc.contributor.authorLukasik, Karolina
dc.contributor.authorGawronska-Kozak, Barbara
dc.contributor.authorGama, Luis
dc.contributor.authorSkarzynski, Dariusz J.
dc.contributor.authorFerreira-Dias, Graça
dc.date.accessioned2022-04-18T14:07:04Z
dc.date.available2022-04-18T14:07:04Z
dc.date.issued2020-05-16
dc.descriptionResearch Areas: Agriculture ; Veterinary Sciences ; Zoologypt_PT
dc.description.abstractAbstract: Neutrophil extracellular traps (NETs) fight endometritis, and elastase (ELA), a protease found in NETs, might induce collagen type I (COL1) accumulation in equine endometrium. Metallopeptidases (MMPs) are involved in extracellular matrix balance. The aim was to evaluate the e ects of ELA and sivelestat (selective elastase inhibitor) on MMP-2 and MMP-9 expression and gelatinolytic activity, as well as the potential inhibitory e ect of sivelestat on ELA-induced COL1 in equine endometrium. Endometrial explants from follicular (FP) and mid-luteal (MLP) phases were treated for 24 or 48 h with ELA, sivelestat, and their combination. Transcripts of COL1A2, MMP2, and MMP9 were evaluated by qPCR; COL1 protein relative abundance by Western blot, and MMP-2 and MMP-9 gelatinolytic activity by zymography. In response to ELA treatment, there was an increase in MMP2 mRNA transcription (24 h) in active MMP-2 (48 h), both in FP, and in MMP9 transcripts in FP (48 h) and MLP (24 h) (p < 0.05). Sivelestat inhibited ELA-induced COL1A2 transcripts in FP (24 h) and MLP (24 h, 48 h) (p < 0.05). The sivelestat inhibitory e ect was detected in MMP9 transcripts in FP at 48 h (p < 0.05), but proteases activity was unchanged. Thus, MMP-2 and MMP-9 might be implicated in endometrium fibrotic response to ELA. In mare endometrium, sivelestat may decrease ELA-induced COL1 deposition and hinder endometrosis development.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAmaral A, Fernandes C, Rebordao MR, Szostek-Mioduchowska A, Lukasik K, Lukasik K, Gawronska-Kozak B, Gama LT, Skarzynski DJ, Ferreira-Dias, G . 2020. The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium. Animals, 10(5):863. Doi: 10.3390/ani10050863pt_PT
dc.identifier.doi10.3390/ani10050863pt_PT
dc.identifier.issn2076-2615
dc.identifier.urihttp://hdl.handle.net/10400.5/24097
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPPN/BIL/2018/1/00250/U/0001pt_PT
dc.relationUIDP/CVT/00276/2020pt_PT
dc.relationNeutrophil extracellular traps and fibrotic cytokines: a challenge in endometrium fibrosis
dc.relation.publisherversionhttps://www.mdpi.com/2076-2615/10/5/863pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEndometrosispt_PT
dc.subjectMarept_PT
dc.subjectElastasept_PT
dc.subjectSivelestatpt_PT
dc.subjectCollagenpt_PT
dc.subjectMetallopeptidasespt_PT
dc.subjectEndometriumpt_PT
dc.subjectNeutrophil extracellular traps (NETs)pt_PT
dc.titleThe In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometriumpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNeutrophil extracellular traps and fibrotic cytokines: a challenge in endometrium fibrosis
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCVT-REP%2F4202%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F101058%2F2014/PT
oaire.citation.conferencePlaceBasel, Switzerlandpt_PT
oaire.citation.titleAnimalspt_PT
oaire.citation.volume10(5):863pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameAmaral
person.familyNameRebordão Cordeiro Simões Crisóstomo
person.familyNameGama
person.familyNameLeitão Ferreira-Dias
person.givenNameAna
person.givenNameMaria Rosa
person.givenNameLuis
person.givenNameGraça Maria
person.identifier.ciencia-idEB18-9420-421B
person.identifier.ciencia-id9515-257A-B35D
person.identifier.ciencia-idC71D-140F-E876
person.identifier.ciencia-idF51C-7F45-C720
person.identifier.orcid0000-0002-0075-262X
person.identifier.orcid0000-0002-4389-445X
person.identifier.orcid0000-0003-3894-3488
person.identifier.orcid0000-0003-0622-6513
person.identifier.ridN-4172-2018
person.identifier.scopus-author-id56394000500
person.identifier.scopus-author-id36027387300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationd1fd322a-70b1-4446-afe0-a5a4b9d1f5fa
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