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The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium
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Orientador(es)
Resumo(s)
Abstract: Neutrophil extracellular traps (NETs) fight endometritis, and elastase (ELA), a protease
found in NETs, might induce collagen type I (COL1) accumulation in equine endometrium.
Metallopeptidases (MMPs) are involved in extracellular matrix balance. The aim was to evaluate
the e ects of ELA and sivelestat (selective elastase inhibitor) on MMP-2 and MMP-9 expression and
gelatinolytic activity, as well as the potential inhibitory e ect of sivelestat on ELA-induced COL1 in
equine endometrium. Endometrial explants from follicular (FP) and mid-luteal (MLP) phases were
treated for 24 or 48 h with ELA, sivelestat, and their combination. Transcripts of COL1A2, MMP2, and
MMP9 were evaluated by qPCR; COL1 protein relative abundance by Western blot, and MMP-2 and
MMP-9 gelatinolytic activity by zymography. In response to ELA treatment, there was an increase in
MMP2 mRNA transcription (24 h) in active MMP-2 (48 h), both in FP, and in MMP9 transcripts in FP
(48 h) and MLP (24 h) (p < 0.05). Sivelestat inhibited ELA-induced COL1A2 transcripts in FP (24 h)
and MLP (24 h, 48 h) (p < 0.05). The sivelestat inhibitory e ect was detected in MMP9 transcripts
in FP at 48 h (p < 0.05), but proteases activity was unchanged. Thus, MMP-2 and MMP-9 might be
implicated in endometrium fibrotic response to ELA. In mare endometrium, sivelestat may decrease
ELA-induced COL1 deposition and hinder endometrosis development.
Descrição
Research Areas: Agriculture ; Veterinary Sciences ; Zoology
Palavras-chave
Endometrosis Mare Elastase Sivelestat Collagen Metallopeptidases Endometrium Neutrophil extracellular traps (NETs)
Contexto Educativo
Citação
Amaral A, Fernandes C, Rebordao MR, Szostek-Mioduchowska A, Lukasik K, Lukasik K, Gawronska-Kozak B, Gama LT, Skarzynski DJ, Ferreira-Dias, G . 2020. The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium. Animals, 10(5):863. Doi: 10.3390/ani10050863
Editora
MDPI