Repository logo
 
Publication

The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium

2021-10-19Journal article Open access
Simple item page
dc.contributor.authorAmaral, Ana
dc.contributor.authorFernandes, Carina
dc.contributor.authorSzostek-Mioduchowska, Anna
dc.contributor.authorLukasik, Karolina
dc.contributor.authorRebordão, Maria Rosa
dc.contributor.authorPinto-Bravo, Pedro
dc.contributor.authorSkarzynski, Dariusz Jan
dc.contributor.authorFerreira-Dias, Graça
dc.date.accessioned2022-01-17T14:52:03Z
dc.date.available2022-01-17T14:52:03Z
dc.date.issued2021-10-19
dc.descriptionResearch Areas: Life Sciences & Biomedicine ; Microbiologypt_PT
dc.description.abstractABSTRACT - Cathepsin G (CAT) is a protease released by neutrophils when forming neutrophil extracellular traps that was already associated with inducing type I collagen (COL1) in equine endometrium in vitro. Endometrosis is a fibrotic condition mainly characterized by COL1 deposition in the equine endometrium. The objective was to evaluate if noscapine (an alkaloid for cough treatment with anti-neoplastic and anti-fibrotic properties) would reduce COL1A2 transcription (evaluated by qPCR) and COL1 protein relative abundance (evaluated by western blot) induced by CAT in equine endometrial explants from follicular and mid-luteal phases treated for 24 or 48 h. The explants treated with CAT increased COL1 expression. Noscapine decreased COL1A2 transcription at both estrous cycle phases, but COL1 relative protein only at the follicular phase, both induced by CAT. Additionally, the noscapine anti-fibrotic action was found to be more effective in the follicular phase. The CAT treatment caused more fibrosis at the longest period of treatment, while noscapine acted better at the shortest time of treatment. Our results showed that noscapine could act as an anti-fibrotic drug in equine endometrosis by inhibiting CAT in vitro. Noscapine offers a new promising therapeutic tool for treating fibrosis as a single non-selective agent to be considered in the future.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAmaral A, Fernandes C, Szostek-Mioduchowska A, Lukasik K, Rebordao MR) 1, 3 Pinto-Bravo, P (Pinto-Bravo, Pedro) 3 Skarzynski, DJ, Ferreira-Dias G. 2021. The Inhibitory Effect of Noscapine on the In Vitro Cathepsin G-Induced Collagen Expression in Equine Endometrium. Life 11(10):1107. Doi: 10.3390/life11101107pt_PT
dc.identifier.doi10.3390/life11101107pt_PT
dc.identifier.eissn2075-1729
dc.identifier.urihttp://hdl.handle.net/10400.5/23107
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPPN/BIL/2018/1/00250/U/0001pt_PT
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relationNeutrophil extracellular traps and fibrotic cytokines: a challenge in endometrium fibrosis
dc.relation.publisherversionhttps://www.mdpi.com/2075-1729/11/10/1107pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEquinept_PT
dc.subjectEndometrosispt_PT
dc.subjectFibrosispt_PT
dc.subjectCollagenpt_PT
dc.subjectCathepsin Gpt_PT
dc.subjectNoscapinept_PT
dc.subjectInhibitionpt_PT
dc.titleThe inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometriumpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardTitleNeutrophil extracellular traps and fibrotic cytokines: a challenge in endometrium fibrosis
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCVT-REP%2F4202%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F101058%2F2014/PT
oaire.citation.conferencePlaceBasel, Switzerlandpt_PT
oaire.citation.titleLifept_PT
oaire.citation.volumev.11(10):1107pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
person.familyNameAmaral
person.familyNameRebordão Cordeiro Simões Crisóstomo
person.familyNameLeitão Ferreira-Dias
person.givenNameAna
person.givenNameMaria Rosa
person.givenNameGraça Maria
person.identifier.ciencia-idEB18-9420-421B
person.identifier.ciencia-id9515-257A-B35D
person.identifier.ciencia-idF51C-7F45-C720
person.identifier.orcid0000-0002-0075-262X
person.identifier.orcid0000-0002-4389-445X
person.identifier.orcid0000-0003-0622-6513
person.identifier.scopus-author-id56394000500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication64ab1595-75c6-4b47-b196-ae1d494e6ca1
relation.isAuthorOfPublicationd1fd322a-70b1-4446-afe0-a5a4b9d1f5fa
relation.isAuthorOfPublication0fb8ec33-7ee1-482b-bdcb-eff773226f90
relation.isAuthorOfPublication.latestForDiscoveryd1fd322a-70b1-4446-afe0-a5a4b9d1f5fa
relation.isProjectOfPublication8563a044-0c86-49a8-a275-8b1bb62cc0d8
relation.isProjectOfPublication43b0e213-22c2-40f3-a25f-ef63761c9f04
relation.isProjectOfPublicationfe9194fb-5087-48c6-81fa-5b0c87af379d
relation.isProjectOfPublication.latestForDiscovery43b0e213-22c2-40f3-a25f-ef63761c9f04

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
The Inhibitory Effect of Noscapine on the In Vitro Cathepsin G-Induced Collagen Expression in Equine Endometrium.pdf
Size:
2.24 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIISA - Artigos em revistas internacionais