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Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer?

2020Journal article Open access
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dc.contributor.authorCarvalho, Ana Sofia
dc.contributor.authorMoraes, Maria Carolina Strano
dc.contributor.authorHyun Na, Chan
dc.contributor.authorFierro-Monti, Ivo
dc.contributor.authorHenriques, Andreia
dc.contributor.authorZahedi, Sara
dc.contributor.authorBodo, Cristian
dc.contributor.authorTranfield, Erin M
dc.contributor.authorSousa, Ana Laura
dc.contributor.authorFarinho, Ana
dc.contributor.authorRodrigues, Luís Vaz
dc.contributor.authorPinto, Paula
dc.contributor.authorBárbara, Cristina
dc.contributor.authorMota, Leonor
dc.contributor.authorAbreu, Tiago Tavares de
dc.contributor.authorSemedo, Júlio
dc.contributor.authorSeixas, Susana
dc.contributor.authorKumar, Prashant
dc.contributor.authorCosta-Silva, Bruno
dc.contributor.authorPandey, Akhilesh
dc.contributor.authorMatthiesen, Rune
dc.date.accessioned2020-11-27T12:33:53Z
dc.date.available2020-11-27T12:33:53Z
dc.date.issued2020
dc.description© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).pt_PT
dc.description.abstractAcellular bronchoalveolar lavage (BAL) proteomics can partially separate lung cancer from non-lung cancer patients based on principal component analysis and multivariate analysis. Furthermore, the variance in the proteomics data sets is correlated mainly with lung cancer status and, to a lesser extent, smoking status and gender. Despite these advances BAL small and large extracellular vehicles (EVs) proteomes reveal aberrant protein expression in paracrine signaling mechanisms in cancer initiation and progression. We consequently present a case-control study of 24 bronchoalveolar lavage extracellular vesicle samples which were analyzed by state-of-the-art liquid chromatography-mass spectrometry (LC-MS). We obtained evidence that BAL EVs proteome complexity correlated with lung cancer stage 4 and mortality within two years´ follow-up (p value = 0.006). The potential therapeutic target DNMT3B complex is significantly up-regulated in tumor tissue and BAL EVs. The computational analysis of the immune and fibroblast cell markers in EVs suggests that patients who deceased within the follow-up period display higher marker expression indicative of innate immune and fibroblast cells (four out of five cases). This study provides insights into the proteome content of BAL EVs and their correlation to clinical outcomes.pt_PT
dc.description.sponsorshipR.M. is supported by Fundação para a Ciência e a Tecnologia (CEEC position, 2019–2025 investigator).This article is a result of the projects (iNOVA4Health—UID/Multi/04462/2013), supported by Lisboa PortugalRegional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through theEuropean Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE2020 Programme and National Funds through FCT—Portuguese Foundation for Science and Technology underthe projects number PTDC/BTM-TEC/30087/2017 and PTDC/BTM-TEC/30088/2017. This work was supported bythe Wellcome Trust/DBT India Alliance Margdarshi Fellowship (grant number IA/M/15/1/502023) awarded to A.P.B.C.-S., M.C.S.C. and C.B. are supported by the Champalimaud Foundation and the EMBO Installation Grant 3921pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCancers 2020, 12(11), 3450pt_PT
dc.identifier.doi10.3390/cancers12113450pt_PT
dc.identifier.eissn2072-6694
dc.identifier.urihttp://hdl.handle.net/10451/45056
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationIA/M/15/1/502023pt_PT
dc.relationDiffuse Large B-cell lymphoma blood based proteome signatures
dc.relation.publisherversionhttps://www.mdpi.com/journal/cancerspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectExtracellular vesiclespt_PT
dc.subjectLung cancerpt_PT
dc.subjectProteomicspt_PT
dc.subjectImmuno oncologypt_PT
dc.subjectBronchoalveolar lavagept_PT
dc.titleIs the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDiffuse Large B-cell lymphoma blood based proteome signatures
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04462%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBTM-TEC%2F30087%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBTM-TEC%2F30088%2F2017/PT
oaire.citation.issue11pt_PT
oaire.citation.startPage3450pt_PT
oaire.citation.titleCancerspt_PT
oaire.citation.volume12pt_PT
oaire.fundingStream5876
oaire.fundingStream3599-PPCDT
oaire.fundingStream9471 - RIDTI
person.familyNamePinto
person.familyNameBárbara
person.givenNamePaula Maria Gonçalves
person.givenNameCristina
person.identifier1330713
person.identifier.ciencia-idF01D-6AFB-DFFD
person.identifier.ciencia-id171F-DE21-B23B
person.identifier.orcid0000-0003-2301-2752
person.identifier.orcid0000-0003-0915-4105
person.identifier.scopus-author-id24074744900
person.identifier.scopus-author-id6701574502
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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