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Physiological, cellular and biochemical thermal stress response of intertidal shrimps with different vertical distributions: Palaemon elegans and Palaemon serratus

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dc.contributor.authorMadeira, Diana
dc.contributor.authorMendonça, Vanessa
dc.contributor.authorDias, Marta
dc.contributor.authorRoma, Joana
dc.contributor.authorCosta, Pedro M.
dc.contributor.authorLarguinho, Miguel
dc.contributor.authorVinagre, Catarina
dc.contributor.authorDiniz, Mário S.
dc.date.accessioned2020-01-19T19:44:35Z
dc.date.available2020-01-19T19:44:35Z
dc.date.issued2015
dc.description.abstractThe ability to cope with high temperature variations is a critical factor in intertidal communities. Two species of intertidal rocky shore shrimps (Palaemon sp.) with different vertical distributions were collected from the Portuguese coast in order to test if they were differentially sensitive to thermal stress. Three distinct levels of biological organization (organismal, biochemical, and cellular) were surveyed. The shrimp were exposed to a constant rate of temperature increase of 1°C.h(-1), starting at 20°C until reaching the CTMax (critical thermal maximum). During heat stress, two biomarkers of protein damage were quantified in the muscle via enzyme-linked immunosorbent assays: heat shock proteins HSP70 (hsp70/hsc70) and total ubiquitin. Muscle histopathological alterations caused by temperature were also evaluated. CTMax values were not significantly different between the congeners (P. elegans 33.4 ± 0.5 °C; P. serratus 33.0 ± 0.5 °C). Biomarker levels did not increase along the temperature trial, but P. elegans (higher intertidal) showed higher amounts of HSP70 and total ubiquitin than P. serratus (lower intertidal). HSP70 and total ubiquitin levels showed a positive significant correlation in both species, suggesting that their association is important in thermal tolerance. Histopathological observations of muscle tissue in P. serratus showed no gross alterations due to temperature but did show localized atrophy of muscle fibers at CTMax. In P. elegans, alterations occurred at a larger scale, showing multiple foci of atrophic muscular fascicles caused by necrotic or autolytic processes. In conclusion, Palaemon congeners displayed different responses to stress at a cellular level, with P. elegans having greater biomarker levels and histopathological alterations.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.cbpa.2014.12.039pt_PT
dc.identifier.issn1095-6433
dc.identifier.urihttp://hdl.handle.net/10451/41112
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1095643315000033pt_PT
dc.subjectHsp70pt_PT
dc.subjectUbiquitinpt_PT
dc.subjectHistopathological changespt_PT
dc.subjectCTMaxpt_PT
dc.subjectCrustaceanpt_PT
dc.subjectHeat stresspt_PT
dc.titlePhysiological, cellular and biochemical thermal stress response of intertidal shrimps with different vertical distributions: Palaemon elegans and Palaemon serratuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage115pt_PT
oaire.citation.startPage107pt_PT
oaire.citation.titleComparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiologypt_PT
oaire.citation.volume183pt_PT
person.familyNameMadeira
person.familyNameAlegria Mendonça
person.familyNameDuarte Dias
person.familyNameRoma
person.familyNameCosta
person.familyNameVinagre
person.givenNameDiana
person.givenNameVanessa Sofia
person.givenNameMarta Andreia
person.givenNameJoana
person.givenNamePedro M.
person.givenNameCatarina
person.identifier440666
person.identifierO-4112-2015
person.identifier106859
person.identifier.ciencia-id0313-5EB2-1A99
person.identifier.ciencia-idAE1E-EECE-EA4B
person.identifier.ciencia-id291F-01D8-E03B
person.identifier.ciencia-id511E-C410-CAEF
person.identifier.ciencia-id281C-7BD4-10C5
person.identifier.ciencia-id3F13-843A-791B
person.identifier.orcid0000-0002-2435-9283
person.identifier.orcid0000-0002-3658-9502
person.identifier.orcid0000-0003-0447-6009
person.identifier.orcid0000-0002-5884-4623
person.identifier.orcid0000-0002-9072-3522
person.identifier.orcid0000-0003-2146-7948
person.identifier.ridN-3228-2013
person.identifier.ridAAL-3670-2021
person.identifier.ridJ-2962-2013
person.identifier.scopus-author-id54401593300
person.identifier.scopus-author-id57201564026
person.identifier.scopus-author-id27169731700
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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