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Estudo de associação de biomarcadores com parâmetros cardiovasculares na psoríase
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Introdução: A psoríase é uma doença imunomediada que acarreta riscos major cardiovasculares. Apesar da manifestação clínica principal ser lesão na pele, a inflamação sistémica da doença associa-se a AVC, EAM, síndrome metabólica, hipertensão arterial, dislipidemia, entre outros. Este trabalho pretende identificar biomarcadores úteis na avaliação da inflamação sistémica e associação com o risco cardiovascular inerente. Os novos biomarcadores sFlt e VEGF-C parecem ser relevantes para estas condições. Prevê-se grande impacto deste trabalho no âmbito da Medicina Geral e Familiar. Objetivos: 1) Identificar novos biomarcadores da mobilização de sódio na pele, como sFlt e VEGF-C, na psoríase e na doença cardiovascular; 2) Determinar relação de índices derivados do hemograma com PASI e doenças cardiovasculares; 3) Determinar o VEGFC e sFLt numa amostra populacional com psoríase; 4) Relação do VEGF-C e sFLt com biomarcadores celulares (hematológicos) e bioquímicos de doenças cardiovasculares. Metodologia: Foram estudados 63 indivíduos com psoríase e colhidas amostras de sangue periférico. O sflt, TNF-alfa e VEGF-C foram determinados com recurso a kits ELISA. Os restantes biomarcadores foram determinados por métodos padronizados. A análise estatística foi realizada no programa SPSS 27.0 tendo os valores p < 0,05 considerados estatisticamente significativos. Resultados: Demonstraram-se com relevância estatística vários biomarcadores. Apesar de não se verificar relação direta significativa, o sFlt e VEGF-C demonstraram relação indireta com o score PASI. Mais estudos são necessários sobre este tema. Conclusão: É possível identificar biomarcadores celulares e bioquímicos que demonstram relevo na avaliação clínica do estadio inflamatório sistémico do doente. Esses biomarcadores demonstram relevo pela acessibilidade e praticidade. Os novos biomarcadores estudados revelam serem úteis, como marcadores indiretos, de inflamação sistémica e possíveis alvos de investigação e terapia no futuro.
Background: Psoriasis is an immune-mediated disease that carries major cardiovascular risks. Despite the main clinical manifestation being the skin lesion, the systemic inflammation of the disease is associated with other conditions such as stroke, myocardial infarction, metabolic syndrome, arterial hypertension, dyslipidemia, among others. This work aims to identify useful biomarkers in the assessment of systemic inflammation and its association with the inherent cardiovascular risk. The new biomarkers sFlt and VEGF-C appear to be relevant for this purpose. A major impact of this work is expected within the scope of General and Family Medicine. Objectives: 1) Identify new biomarkers of sodium mobilization in the skin, such as sFlt and VEGF-C, in psoriasis and cardiovascular disease; 2) Determine the relationship of blood count indices with PASI and cardiovascular diseases; 3) Determine VEGF-C and sFLt in a population with psoriasis; 4) Access the relation of VEGF-C and sFLt with cellular (hematological) and biochemical biomarkers of cardiovascular diseases. Methodology: 63 individuals with psoriasis were studied and peripheral blood samples were collected. Sflt, TNF-alpha and VEGF-C were determined using ELISA kits. The remaining biomarkers were determined by standard methods. Statistical analysis was performed using the SPSS 27.0 program, with p values < 0,05 considered statistically significant. Results: Numerous biomarkers were demonstrated with statistical relevance. Although there was no significant direct relationship, sFlt and VEGF-C showed an indirect relationship with the PASI score. More studies are needed on this topic. Conclusion: It is possible to identify cellular and biochemical biomarkers that show relevance in clinical evaluation of the patient's systemic inflammatory situation. These biomarkers have demonstrated relevance for its accessibility and practicality. The new biomarkers studied prove to be useful, as indirect markers of systemic inflammation, and possible targets for research and therapy, in the future.
Background: Psoriasis is an immune-mediated disease that carries major cardiovascular risks. Despite the main clinical manifestation being the skin lesion, the systemic inflammation of the disease is associated with other conditions such as stroke, myocardial infarction, metabolic syndrome, arterial hypertension, dyslipidemia, among others. This work aims to identify useful biomarkers in the assessment of systemic inflammation and its association with the inherent cardiovascular risk. The new biomarkers sFlt and VEGF-C appear to be relevant for this purpose. A major impact of this work is expected within the scope of General and Family Medicine. Objectives: 1) Identify new biomarkers of sodium mobilization in the skin, such as sFlt and VEGF-C, in psoriasis and cardiovascular disease; 2) Determine the relationship of blood count indices with PASI and cardiovascular diseases; 3) Determine VEGF-C and sFLt in a population with psoriasis; 4) Access the relation of VEGF-C and sFLt with cellular (hematological) and biochemical biomarkers of cardiovascular diseases. Methodology: 63 individuals with psoriasis were studied and peripheral blood samples were collected. Sflt, TNF-alpha and VEGF-C were determined using ELISA kits. The remaining biomarkers were determined by standard methods. Statistical analysis was performed using the SPSS 27.0 program, with p values < 0,05 considered statistically significant. Results: Numerous biomarkers were demonstrated with statistical relevance. Although there was no significant direct relationship, sFlt and VEGF-C showed an indirect relationship with the PASI score. More studies are needed on this topic. Conclusion: It is possible to identify cellular and biochemical biomarkers that show relevance in clinical evaluation of the patient's systemic inflammatory situation. These biomarkers have demonstrated relevance for its accessibility and practicality. The new biomarkers studied prove to be useful, as indirect markers of systemic inflammation, and possible targets for research and therapy, in the future.
Descrição
Trabalho Final do Curso de Mestrado Integrado em Medicina, Faculdade de Medicina, Universidade de Lisboa, 2022
Palavras-chave
Psoríase Inflamação Biomarcadores Comorbilidades Prognóstico