Publication
Complex Polysaccharides as PCR Inhibitors in Feces: Helicobacter pylori Model
| dc.contributor.author | Monteiro, Lurdes | |
| dc.contributor.author | Bonnemaison, Dominique | |
| dc.contributor.author | Vekris, Antoine | |
| dc.contributor.author | Petry, Klaus G. | |
| dc.contributor.author | Bonnet, Jacques | |
| dc.contributor.author | Vidal, Rui | |
| dc.contributor.author | Cabrita, José | |
| dc.contributor.author | Mégraud, Francis | |
| dc.date.accessioned | 2017-07-20T14:57:19Z | |
| dc.date.available | 2017-07-20T14:57:19Z | |
| dc.date.issued | 1997 | |
| dc.description.abstract | A model was developed to study inhibitors present in feces which prevent the use of PCR for the detection of Heticobacterpylori. A DNA fragment amplified with the same primers as H. pytori was used to spike samples before extraction by a modified QIAamp tissue method. Inhibitors, separated on an Ultrogel AcA44 column, were characterized. Inhibitors in feces are complex polysaccharides possibly originating from vegetable material in the diet. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Journal of Clinical Microbiology,1997;35(4):995-998 | pt_PT |
| dc.identifier.issn | 0095-1137 | |
| dc.identifier.issn | 1098-660X | |
| dc.identifier.uri | http://hdl.handle.net/10451/28442 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.relation.publisherversion | http://jcm.asm.org/content/35/4/995.full.pdf+html | pt_PT |
| dc.title | Complex Polysaccharides as PCR Inhibitors in Feces: Helicobacter pylori Model | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 998 | pt_PT |
| oaire.citation.issue | 4 | pt_PT |
| oaire.citation.startPage | 995 | pt_PT |
| oaire.citation.title | Journal of Clinical Microbiology | pt_PT |
| oaire.citation.volume | 35 | pt_PT |
| rcaap.rights | restrictedAccess | pt_PT |
| rcaap.type | article | pt_PT |