Complex Polysaccharides as PCR Inhibitors in Feces: Helicobacter pylori Model

Monteiro, Lurdes; Bonnemaison, Dominique; Vekris, Antoine; Petry, Klaus G.; Bonnet, Jacques; Vidal, Rui; Cabrita, José; Mégraud, Francis

http://hdl.handle.net/10451/28442

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Authors

Monteiro, Lurdes

Bonnemaison, Dominique

Abstract(s)

A model was developed to study inhibitors present in feces which prevent the use of PCR for the detection of Heticobacterpylori. A DNA fragment amplified with the same primers as H. pytori was used to spike samples before extraction by a modified QIAamp tissue method. Inhibitors, separated on an Ultrogel AcA44 column, were characterized. Inhibitors in feces are complex polysaccharides possibly originating from vegetable material in the diet.