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Selective antibacterial activity of the cationic peptide PaDBS1R6 against Gram-negative bacteria

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dc.contributor.authorFensterseifer, Isabel C. M.
dc.contributor.authorFelício, Mário Romão
dc.contributor.authorAlves, Eliane S. F.
dc.contributor.authorCardoso, Marlon H.
dc.contributor.authorTorres, Marcelo D. T.
dc.contributor.authorMatos, Carolina O.
dc.contributor.authorSilva, Osmar N.
dc.contributor.authorLu, Timothy K.
dc.contributor.authorFreire, Maurício V.
dc.contributor.authorNeves, Natan C.
dc.contributor.authorGonçalves, Sónia
dc.contributor.authorLião, Luciano M.
dc.contributor.authorSantos, Nuno C.
dc.contributor.authorPorto, William F.
dc.contributor.authorde la Fuente-Nunez, Cesar
dc.contributor.authorFranco, Octavio L.
dc.date.accessioned2022-05-12T16:00:45Z
dc.date.available2022-05-12T16:00:45Z
dc.date.issued2019
dc.description© 2019 Elsevier B.V. All rights reserved.pt_PT
dc.description.abstractInfections caused by Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa foremost among them, constitute a major worldwide health problem. Bioinformatics methodologies are being used to rationally design new antimicrobial peptides, a potential alternative for treating these infections. One of the algorithms used to develop antimicrobial peptides is the Joker, which was used to design the peptide PaDBS1R6. This study evaluates the antibacterial activities of PaDBS1R6 in vitro and in vivo, characterizes the peptide interaction to target membranes, and investigates the PaDBS1R6 structure in contact with mimetic vesicles. Moreover, we demonstrate that PaDBS1R6 exhibits selective antimicrobial activity against Gram-negative bacteria. In the presence of negatively charged and zwitterionic lipids the structural arrangement of PaDBS1R6 transits from random coil to α-helix, as characterized by circular dichroism. The tertiary structure of PaDBS1R6 was determined by NMR in zwitterionic dodecylphosphocholine (DPC) micelles. In conclusion, PaDBS1R6 is a candidate for the treatment of nosocomial infections caused by Gram-negative bacteria, as template for producing other antimicrobial agents.pt_PT
dc.description.sponsorshipThis work was supported by Fundação para a Ciência e a Tecnologia – Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal) project PTDC/BBB-BQB/3494/2014, Marie Skłodowska-Curie Research and Innovation Staff Exchange (MSCA-RISE, European Union) project INPACT (call H2020-MSCA-RISE-2014, grant agreement 644167), Ramon Areces Foundation (to CFN), DTRA (HDTRA1-15-1-0050 to TKL), Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP #2016/24413-0 to MDTT), Fundação de Amparo à Pesquisa do Estado de Goiás (FAPEG # 201710267000062 to ESFA) and Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF). MRF acknowledges FCT-MCTES fellowship SFRH/BD/100517/2014. ONS holds a postdoctoral scholarship from National Council of Technological and Scientific Development (CNPq) and Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT) – Brazil [300583/2016-8]. MHC acknowledges fellowship 141518/2015-4 (CNPq) and 88881.134423/2016-01 (CAPES).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBiochim Biophys Acta Biomembr. 2019 Jul 1;1861(7):1375-1387pt_PT
dc.identifier.doi10.1016/j.bbamem.2019.03.016pt_PT
dc.identifier.eissn1879-2642
dc.identifier.issn0005-2736
dc.identifier.urihttp://hdl.handle.net/10451/52958
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationBroad-spectrum antiviral peptides against respiratory viruses
dc.relationInnovative peptides against cancer and pathogenic bacteria, with advances in science, biopharmaceutical drug development, product market targeting, training , and communication.
dc.relationPhotoswitchable peptides as new antimicrobial and anticancer agents
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/biochimica-et-biophysica-acta-bba-biomembranespt_PT
dc.subjectAntibiotic resistancept_PT
dc.subjectAntimicrobial peptide (AMP)pt_PT
dc.subjectDrug designpt_PT
dc.subjectEscherichia colipt_PT
dc.subjectJoker algorithmpt_PT
dc.subjectPaDBS1R6pt_PT
dc.subjectPseudomonas aeruginosapt_PT
dc.titleSelective antibacterial activity of the cationic peptide PaDBS1R6 against Gram-negative bacteriapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberPTDC/BBB-BQB/3494/2014
oaire.awardNumber644167
oaire.awardNumberSFRH/BD/100517/2014
oaire.awardTitleBroad-spectrum antiviral peptides against respiratory viruses
oaire.awardTitleInnovative peptides against cancer and pathogenic bacteria, with advances in science, biopharmaceutical drug development, product market targeting, training , and communication.
oaire.awardTitlePhotoswitchable peptides as new antimicrobial and anticancer agents
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBBB-BQB%2F3494%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/644167/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F100517%2F2014/PT
oaire.citation.endPage1387pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage1375pt_PT
oaire.citation.titleBiochimica et Biophysica Acta (BBA) - Biomembranespt_PT
oaire.citation.volume1861pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamH2020
oaire.fundingStreamOE
person.familyNameRomão Felício
person.familyNameAbreu
person.familyNameSantos
person.givenNameMário
person.givenNameSónia
person.givenNameNuno
person.identifier384205
person.identifier.ciencia-idF811-74AE-412A
person.identifier.ciencia-idCD13-5E3A-A3C5
person.identifier.orcid0000-0002-9398-2140
person.identifier.orcid0000-0003-2903-4282
person.identifier.orcid0000-0002-0580-0475
person.identifier.ridJ-8517-2014
person.identifier.ridH-9482-2017
person.identifier.ridN-7248-2013
person.identifier.scopus-author-id57144367400
person.identifier.scopus-author-id35602210000
person.identifier.scopus-author-id55940818300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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