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In vitro screening for acetylcholinesterase inhibition and antioxidant activity of Quercus suber cork and corkback extracts
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Orientador(es)
Resumo(s)
Purpose. Acetylcholinesterase (AChE) inhibitors are used to treat Alzheimer’s patients because they enhance cholinergic
neurotransmission. It is urgent to find new and efficient inhibitors from natural sources, highly bioavailable with low or no
toxicity. +e plant kingdom is extremely rich in a variety of compounds that are potent AChE inhibitors: flavonoids and other
phenolic compounds have been recognized as promising Alzheimer’s treatment agents. In this study, in vitro acetylcholinesterase
inhibition, antioxidant activities, and total flavonoid and phenolic contents of ethanol-water extracts from Quercus suber cork and
corkback were evaluated. Methods. +e acetylcholinesterase activity was determined by a colorimetric assay based on Ellman’s
methodology. +e Folin–Ciocalteu colorimetric method was used for total phenolic content determination and the aluminium
chloride method for the determination of total flavonoid content. Antioxidant activity assays were performed using the DPPH and
FRAP assays. Results. +e acetylcholinesterase inhibitory activity from Q. suber cork and corkback ethanol-water extracts was as
follows: 62% inhibition with corkback extracts over 0.5 mg/mL and around 49% inhibition in cork extracts over 1.0 mg/mL
extracts’ concentration. Regarding the DPPH radical scavenging activity, the concentrations of cork and corkback ethanol-water
extracts required for 50% DPPH inhibition (IC50) were 3.2 μg/mL and 4.0–5.2 μg/mL. Corkback extracts are less effective than
Trolox standard (3.2 μg/mL) but cork extracts showed the same free radical scavenging activity compared to Trolox. Cork and
corkback extracts have antioxidant power of 750.9–775.4 mg TEAC/g extract and 1051.2–2052.4 mg TEAC/g extract, respectively,
which are significantly higher than the ones obtained with Trolox: 19.6–21.0 mg TEAC/g extract (cork assays) and 57.4–66.3 mg
TEAC/g extract (corkback assays). +e amounts of total phenolic (TPC) and flavonoid (TFC) compounds were 8.7–32.3 mg GAE/
g and 4.8–10.7 mg CE/g dry mass for cork and 5.4–5.7 mg GAE/g and 42.5 mg CE/g dry mass for corkback extracts, respectively,
using catechin (CE) and GAE (gallic acid) as standards. Conclusion. +ese findings demonstrate the remarkable potential of these
extracts as valuable source of antioxidants with interesting acetylcholinesterase inhibitory activity
Descrição
Research Article
Palavras-chave
cork corkback extracts antioxidant activity acetylcholinesterase
Contexto Educativo
Citação
Evidence-Based Complementary and Alternative Medicine Volume 2020, Article ID 3825629, 8 pages
Editora
Hindawi