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Microbial contamination in the coffee industry: an occupational menace besides a food safety concern?

2022Artigo científico Acesso aberto
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dc.contributor.authorViegas, Carla
dc.contributor.authorGomes, Bianca
dc.contributor.authorOliveira, Filipe
dc.contributor.authorDias, Marta
dc.contributor.authorCervantes, Renata
dc.contributor.authorPena, Pedro
dc.contributor.authorGomes, Anita Q.
dc.contributor.authorCaetano, Liliana Aranha
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorde Andrade, Ednilton Tavares
dc.contributor.authorViegas, Susana
dc.date.accessioned2024-11-19T13:58:00Z
dc.date.available2024-11-19T13:58:00Z
dc.date.issued2022
dc.description© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).pt_PT
dc.description.abstractRespiratory abnormalities among workers at coffee roasting and packaging facilities have already been reported; however, little is known about microbiological contamination inside coffee production facilities. This study intends to assess the microbial contamination (fungi and bacteria) in two coffee industries from Brazil with a multi-approach protocol for sampling and for subsequent analyses using four main sources of samples: filtering respiratory protection devices (FRPD) used by workers, settled dust, electrostatic dust cloths (EDC) and coffee beans. The fungal contamination in the assessed industries was also characterized through the molecular detection of toxigenic species and antifungal resistance. Total bacteria contamination presented the highest values in FRPD collected from both industries (7.45 × 104 CFU·m-2; 1.09 × 104 CFU·m-2). Aspergillus genera was widespread in all the environmental samples collected and sections with clinical relevance (Fumigati) and with toxigenic potential (Nigri and Circumdati) were recovered from FRPD. Circumdati section was observed in 4 mg/mL itraconazole. Sections Circumdati (EDC, coffee beans and settled dust) and Nidulantes (EDC, coffee beans and FRPD) were detected by qPCR. Some of the targeted Aspergillus sections that have been identified microscopically were not detected by qPCR and vice-versa. Overall, this study revealed that microbial contamination is a potential occupational risk in the milling stage and should be tackled when assessing exposure and performing risk assessment. In addition, a multi-sampling campaign should be the approach to follow when assessing microbial contamination and FRPD should be included in this campaign. Occupational exposure to mycotoxins should be considered due to high fungal diversity and contamination. A One Health approach should address these issues in order to prevent consumption of coffee crops and beans infected by fungi and, more specifically, to avoid widespread azole resistance.pt_PT
dc.description.sponsorshipThis research was funded by H&TRC—Health & Technology Research Center, ESTeSL—Escola Superior de Tecnologia e Saúde, Instituto Politécnico de Lisboa, NOVA National School of Public Health, Public Health Research Centre, Universidade Nova de Lisboa, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001 and by FCT—Fundação para a Ciência e a Tecnologia, I.P. (Portugal), within the scope of the PhD Grant UI/BD/151431/2021.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInt J Environ Res Public Health. 2022 Oct 18;19(20):13488pt_PT
dc.identifier.doi10.3390/ijerph192013488pt_PT
dc.identifier.eissn1660-4601
dc.identifier.issn1661-7827
dc.identifier.urihttp://hdl.handle.net/10400.5/95431
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationUI/BD/151431/2021pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/journal/ijerphpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAspergilluspt_PT
dc.subjectOne Health approachpt_PT
dc.subjectAzole resistancept_PT
dc.subjectMilling stagept_PT
dc.subjectMulti-approach for sampling and analysespt_PT
dc.titleMicrobial contamination in the coffee industry: an occupational menace besides a food safety concern?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue20pt_PT
oaire.citation.titleInternational Journal of Environmental Research and Public Healthpt_PT
oaire.citation.volume19pt_PT
person.familyNameGomes
person.familyNameCaetano
person.familyNameCarolino
person.familyNameViegas
person.givenNameAnita
person.givenNameLiliana Aranha
person.givenNameElisabete
person.givenNameSusana
person.identifier166296
person.identifier248817
person.identifier.ciencia-id4B10-E015-52B7
person.identifier.ciencia-id9716-9DAC-532A
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0002-3348-0448
person.identifier.orcid0000-0003-1496-2609
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0003-1015-8760
person.identifier.ridC-3580-2014
person.identifier.ridO-4292-2016
person.identifier.ridF-1012-2015
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id7202386033
person.identifier.scopus-author-id55490838100
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id35270591500
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication329776ed-aea9-4b80-810c-50e80fc675f9
relation.isAuthorOfPublication928eb371-e67a-46b6-b261-3d68dca35754
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relation.isAuthorOfPublication.latestForDiscovery928eb371-e67a-46b6-b261-3d68dca35754

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