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Publication
Vitis vinifera secondary metabolism as affected by sulfate depletion: diagnosis through phenylpropanoid pathway genes and metabolites
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Advisor(s)
Abstract(s)
Grapevine (Vitis vinifera L.) is rich in phenylpropanoid compounds, namely flavonoids and stilbenes
which, present in most tissues, are described as antioxidants and known to accumulate in response to
biotic and abiotic stress. Grapevine is then a choice model for studying the interplay between the
phenylpropanoid pathway and nutrient deficiency. Here we report the response to sulfur deficiency ( S)
of flavonoids and stilbenes biosynthetic pathways in chlorophyll tissues (plantlets) and cell culture.
Anthocyanins and trans-resveratrol accumulated in plantlets and trans-resveratrol glucoside in cell
cultures in response to sulfur deficiency, while a significant decrease in chlorophyll was observed in S
plantlets. The up-regulation of chalcone synthase gene and the downstream flavonoid biosynthesis genes
dihydroflavonol reductase and anthocyanidin synthase matched the accumulation of anthocyanins in S
V. vinifera plantlets. The mRNA level of stilbene synthase gene(s) was correlated tightly with the increase
in trans-resveratrol and trans-resveratrol glucoside levels, respectively in S plantlets and cell cultures.
As a whole, the present study unveil that V. vinifera under sulfur deficiency allocates resources to the
phenylpropanoid pathway, probably consecutive to inhibition of protein synthesis, which can be advantageous
to resist against oxidative stress symptoms evoked by S conditions
Description
Keywords
anthocyanins chalcone synthase stilbenes stilbenes synthase sulfur deficiency Vitis vinifera
Pedagogical Context
Citation
"Plant Physiology and Biochemistry". ISSN 0981-9428. 66 (2013) p. 118-126