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Comparing matrix-assisted laser desorption ionization–time of flight mass spectrometry and phenotypic and molecular methods for identification of species within the Streptococcus anginosus group

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dc.contributor.authorArinto-Garcia, Raquel
dc.contributor.authorPinho, Marcos Daniel
dc.contributor.authorCarrico, Joao Andre
dc.contributor.authorCristino, José Melo
dc.contributor.authorRamirez, Mário
dc.date.accessioned2022-03-08T17:43:10Z
dc.date.available2022-03-08T17:43:10Z
dc.date.issued2015
dc.description© 2015 American Society for Microbiology.pt_PT
dc.description.abstractThe heterogeneity of members of the Streptococcus anginosus group (SAG) has traditionally hampered their correct identification. Recently, the group was subdivided into 6 taxa whose prevalence among human infections is poorly described. We evaluated the accuracy of the Rapid ID32 Strep test, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and a PCR multiplex method to identify 212 SAG isolates recovered from human infections to the species and subspecies level by using multilocus sequence analysis (MLSA) as the gold standard. We also determined the antimicrobial susceptibilities of the isolates. Representatives of all SAG taxa were found among our collection. MALDI-TOF MS and the Rapid ID32 Strep test correctly identified 92% and 68% of the isolates to the species level, respectively, but showed poor performance at the subspecies level, and the latter was responsible for major identification errors. The multiplex PCR method results were in complete agreement with the MLSA identifications but failed to distinguish the subspecies Streptococcus constellatus subsp. pharyngis and S. constellatus subsp. viborgensis. A total of 145 MLSA sequence types were present in our collection, indicating that within each taxon a number of different lineages are capable of causing infection. Significant antibiotic resistance was observed only to tetracycline, erythromycin, and clindamycin and was present in most taxa. MALDI-TOF MS is a reliable method for routine SAG species identification, while the need for identification to the subspecies level is not clearly established.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Clin Microbiol . 2015 Nov;53(11):3580-3588pt_PT
dc.identifier.doi10.1128/JCM.01892-15pt_PT
dc.identifier.eissn1098-660X
dc.identifier.issn0095-1137
dc.identifier.urihttp://hdl.handle.net/10451/51657
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relation.publisherversionhttps://journals.asm.org/journal/jcmpt_PT
dc.titleComparing matrix-assisted laser desorption ionization–time of flight mass spectrometry and phenotypic and molecular methods for identification of species within the Streptococcus anginosus grouppt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3588pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage3580pt_PT
oaire.citation.titleJournal of Clinical Microbiologypt_PT
oaire.citation.volume53pt_PT
person.familyNameArinto-Garcia
person.familyNameCaetano Borges de Pinho
person.familyNameCarrico
person.familyNameMelo Cristino
person.familyNameRamos de Almeida Ramirez
person.givenNameRaquel
person.givenNameMarcos Daniel
person.givenNameJoao
person.givenNameJosé
person.givenNameMário Nuno
person.identifier391563
person.identifierB-4993-2008
person.identifier.ciencia-id6D11-22FF-CFD5
person.identifier.ciencia-idF311-F1CD-9224
person.identifier.ciencia-id871E-6AD6-F37C
person.identifier.ciencia-id9C1C-F2A2-4226
person.identifier.orcid0000-0003-1050-335X
person.identifier.orcid0000-0002-4298-0014
person.identifier.orcid0000-0002-5274-2722
person.identifier.orcid0000-0001-8643-1722
person.identifier.orcid0000-0002-4084-6233
person.identifier.ridA-7367-2008
person.identifier.ridH-3726-2013
person.identifier.scopus-author-id8266333200
person.identifier.scopus-author-id7004053640
person.identifier.scopus-author-id7201568476
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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