Publicação
Myofibril contraction and crosslinking drive nuclear movement to the periphery of skeletal muscle
| dc.contributor.author | Roman, William | |
| dc.contributor.author | Martins, João P. | |
| dc.contributor.author | Carvalho, Filomena A. | |
| dc.contributor.author | Voituriez, Raphael | |
| dc.contributor.author | Abella, Jasmine V. G. | |
| dc.contributor.author | Santos, Nuno C. | |
| dc.contributor.author | Cadot, Bruno | |
| dc.contributor.author | Way, Michael | |
| dc.contributor.author | Gomes, Edgar | |
| dc.date.accessioned | 2018-05-07T15:45:27Z | |
| dc.date.available | 2018-05-07T15:45:27Z | |
| dc.date.issued | 2017 | |
| dc.description | © 2017 Macmillan Publishers Limited, part of Springer Nature. All rights reserved. | pt_PT |
| dc.description.abstract | Nuclear movements are important for multiple cellular functions, and are driven by polarized forces generated by motor proteins and the cytoskeleton. During skeletal myofibre formation or regeneration, nuclei move from the centre to the periphery of the myofibre for proper muscle function. Centrally located nuclei are also found in different muscle disorders. Using theoretical and experimental approaches, we demonstrate that nuclear movement to the periphery of myofibres is mediated by centripetal forces around the nucleus. These forces arise from myofibril contraction and crosslinking that 'zip' around the nucleus in combination with tight regulation of nuclear stiffness by lamin A/C. In addition, an Arp2/3 complex containing Arpc5L together with γ-actin is required to organize desmin to crosslink myofibrils for nuclear movement. Our work reveals that centripetal forces exerted by myofibrils squeeze the nucleus to the periphery of myofibres. | pt_PT |
| dc.description.sponsorship | This work was supported by the European Research Council (E.R.G.), EMBO installation (E.R.G.), the myograd PhD programme (W.R.), AIM France (W.R., B.C., E.R.G.), LISBOA-01-0145-FEDER-007391 co-funded by FEDER through POR Lisboa 2020—Programa Operacional Regional de Lisboa, do PORTUGAL 2020 (E.R.G.), and Fundação para a Ciência e a Tecnologia (E.R.G.). M.W. and J.V.G.A. were supported by the Francis Crick Institute, which receives its core funding from Cancer Research UK (FC001209), the UK Medical Research Council (FC001209) and the Wellcome Trust (FC001209), as well as by postdoctoral fellowships from FRQS (Fonds de recherche du Québec—Santé), EMBO and the Canadian Institutes of Health Research (CIHR) to J.V.G.A. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Nature Cell Biology Volume 19 | Number 10 | October 2017 | pt_PT |
| dc.identifier.doi | DOI: 10.1038/ncb3605 | pt_PT |
| dc.identifier.issn | 1465-7392 | |
| dc.identifier.uri | http://hdl.handle.net/10451/33205 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Nature Publishing Group | pt_PT |
| dc.relation | LISBOA-01-0145-FEDER-007391 | pt_PT |
| dc.relation | Cellular Signalling and Cytoskeletal Function | |
| dc.relation.publisherversion | https://www.nature.com/ncb/ | pt_PT |
| dc.title | Myofibril contraction and crosslinking drive nuclear movement to the periphery of skeletal muscle | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Cellular Signalling and Cytoskeletal Function | |
| oaire.awardURI | info:eu-repo/grantAgreement/WT//FC001209 | |
| oaire.citation.endPage | 1201 | pt_PT |
| oaire.citation.issue | 10 | pt_PT |
| oaire.citation.startPage | 1189 | pt_PT |
| oaire.citation.title | Nature Cell Biology | pt_PT |
| oaire.citation.volume | 19 | pt_PT |
| project.funder.identifier | http://doi.org/10.13039/100010269 | |
| project.funder.name | Wellcome Trust | |
| rcaap.rights | restrictedAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isProjectOfPublication | 2ea88d1b-1f66-4bcb-8e49-e3fc3d60b8b8 | |
| relation.isProjectOfPublication.latestForDiscovery | 2ea88d1b-1f66-4bcb-8e49-e3fc3d60b8b8 |