Logo do repositório
 
Publicação

The effects of improving low dietary protein utilization on the proteome of lamb tissues

2020Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorRibeiro, D.M.
dc.contributor.authorPlanchon, S.
dc.contributor.authorLeclercq, C.C.
dc.contributor.authorDentinho, M.T.P.
dc.contributor.authorBessa, R.J.B.
dc.contributor.authorSantos-Silva, J.
dc.contributor.authorPaulos, K.
dc.contributor.authorJerónimo, E.
dc.contributor.authorRenault, J.
dc.contributor.authorAlmeida, A.M.
dc.date.accessioned2021-09-22T10:10:35Z
dc.date.available2021-09-22T10:10:35Z
dc.date.issued2020
dc.description.abstractCistus ladanifer L. is a common shrub endemic to the Mediterranean region with high levels of condensed tannins (CT). CT form complexes with dietary protein resisting microbial degradation in the rumen, which enhances dietary protein utilization in ruminant diets. The objective of this study was to evaluate the utilization of CT in the diet of lambs on the proteomes of muscle, hepatic and adipose tissues. Twenty-four Merino Branco ram lambs were randomly allocated to three treatments (n = 8): C – control (160 g crude protein (CP)) per kg DM, RP – reduced protein (120 g CP/kg DM); and RPCT – reduced protein (120 g CP/kg DM) treated with CT extract. At the end of the trial, lambs were slaughtered and the longissimus lumborum muscle, hepatic and peri-renal adipose tissues sampled. A two-way approach was used for proteomic analysis: 2D-DIGE and nanoLC-MS. In the muscle, C lambs had lower abundance proteins that partake in the glycolysis pathway than the lambs of other treatments. Control lambs had lower abundance of Fe-carrying proteins in the hepatic tissue than RP and RPCT lambs. The latter lambs had highest abundance of hepatic flavin reductase. In the adipose tissue, C lambs had lowest abundance of fatty-acid synthasept_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationRibeiro D.M., Planchon S., Leclercq C.C., Dentinho M.T.P., Bessa R.J.B., Santos-Silva J., Paulos K., Jerónimo E., Renaut J., Almeida A.M. 2020. The effects of improving low dietary protein utilization on the proteome of lamb tissues. Journal of Proteomics 223: 103798pt_PT
dc.identifier.doihttps://doi.org/10.1016/j.jprot.2020.103798pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/21902
dc.language.isoengpt_PT
dc.relationALT20-03-0145-FEDER-000023pt_PT
dc.relationLinking Landscape, Environment, Agriculture and Food
dc.subjectlamb tissuespt_PT
dc.subjectproteomept_PT
dc.subjectdietary proteinpt_PT
dc.titleThe effects of improving low dietary protein utilization on the proteome of lamb tissuespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleLinking Landscape, Environment, Agriculture and Food
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/74495/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAGR%2F04129%2F2013/PT
oaire.citation.titleJournal of Proteomicspt_PT
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication2343b198-18c9-4a7d-89ee-0af634c622d2
relation.isProjectOfPublicationf8f00f10-9d6c-4a8e-875e-7eb9b5281338
relation.isProjectOfPublication.latestForDiscoveryf8f00f10-9d6c-4a8e-875e-7eb9b5281338

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
REP-LEAF-AAlmeida-4 Ribeiro.pdf
Tamanho:
1.82 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

ISA - Artigos em Revistas Internacionais