Publicação
Astrocytes in paper chips and their interaction with hybrid vesicles
| dc.contributor.author | Abild Meyer, Cathrine | |
| dc.contributor.author | De Dios Andres, Paula | |
| dc.contributor.author | Brodszkij, Edit | |
| dc.contributor.author | Westensee, Isabella N. | |
| dc.contributor.author | Lyons, Joseph | |
| dc.contributor.author | Vaz, Sandra H. | |
| dc.contributor.author | Städler, Brigitte | |
| dc.date.accessioned | 2023-02-03T15:09:37Z | |
| dc.date.available | 2023-02-03T15:09:37Z | |
| dc.date.issued | 2022 | |
| dc.description | © 2022 The Authors. Advanced Biology published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | pt_PT |
| dc.description.abstract | The role of astrocytes in brain function has received increased attention lately due to their critical role in brain development and function under physiological and pathophysiological conditions. However, the biological evaluation of soft material nanoparticles in astrocytes remains unexplored. Here, the interaction of crosslinked hybrid vesicles (HVs) and either C8-D1A astrocytes or primary astrocytes cultured in polystyrene tissue culture or floatable paper-based chips is investigated. The amphiphilic block copolymer poly(cholesteryl methacrylate)-block-poly(2-carboxyethyl acrylate) (P1) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine lipids are used for the assembly of HVs with crosslinked membranes. The assemblies show no short-term toxicity towards the C8-D1A astrocytes and the primary astrocytes, and both cell types internalize the HVs when cultured in 2D cell culture. Further, it is demonstrated that both the C8-D1A astrocytes and the primary astrocytes could mature in paper-based chips with preserved calcium signaling and glial fibrillary acidic protein expression. Last, it is confirmed that both types of astrocytes could internalize the HVs when cultured in paper-based chips. These findings lay out a fundamental understanding of the interaction between soft material nanoparticles and astrocytes, even when primary astrocytes are cultured in paper-based chips offering a 3D environment. | pt_PT |
| dc.description.sponsorship | This project was supported by the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement No. 818890), the Lundbeck Foundation (R346-2020-1617), and the Carlsberg Foundation (CF 20–0418). The HRM Queen Margrethe II's travel grant (C.A.M.) is acknowledged for support. T | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Adv Biol (Weinh). 2023 Jan;7(1):e2200209 | pt_PT |
| dc.identifier.doi | 10.1002/adbi.202200209 | pt_PT |
| dc.identifier.eissn | 2701-0198 | |
| dc.identifier.uri | http://hdl.handle.net/10451/56139 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Wiley | pt_PT |
| dc.relation | Hepatocytes-Like Microreactors for Liver Tissue Engineering | |
| dc.relation.publisherversion | https://onlinelibrary.wiley.com/journal/27010198 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Astrocytes | pt_PT |
| dc.subject | Calcium signalling | pt_PT |
| dc.subject | Hybrid vesicles | pt_PT |
| dc.subject | Paper-based chips | pt_PT |
| dc.title | Astrocytes in paper chips and their interaction with hybrid vesicles | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardNumber | 818890 | |
| oaire.awardTitle | Hepatocytes-Like Microreactors for Liver Tissue Engineering | |
| oaire.awardURI | info:eu-repo/grantAgreement/EC/H2020/818890/EU | |
| oaire.citation.issue | 1 | pt_PT |
| oaire.citation.title | Advanced Biology | pt_PT |
| oaire.citation.volume | 7 | pt_PT |
| oaire.fundingStream | H2020 | |
| person.familyName | Henriques Vaz | |
| person.givenName | Sandra Cristina | |
| person.identifier.ciencia-id | 0E1C-952D-61BE | |
| person.identifier.orcid | 0000-0003-4258-9397 | |
| project.funder.identifier | http://doi.org/10.13039/501100008530 | |
| project.funder.name | European Commission | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | b282e0fa-69eb-4079-8752-b7dd914f7745 | |
| relation.isAuthorOfPublication.latestForDiscovery | b282e0fa-69eb-4079-8752-b7dd914f7745 | |
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