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Characterization of the canine CD20 as a therapeutic target for comparative passive immunotherapy

2022-02-17Journal article Open access
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dc.contributor.authorDias, Joana N. R.
dc.contributor.authorAlmeida, André
dc.contributor.authorS. André, Ana
dc.contributor.authorAguiar, Sandra I
dc.contributor.authorBule, Pedro
dc.contributor.authorNogueira, Sara
dc.contributor.authorOliveira, Soraia S
dc.contributor.authorCarrapiço, Belmira
dc.contributor.authorGil, Solange
dc.contributor.authorTavares, Luis
dc.contributor.authorAires Da Silva, Frederico
dc.date.accessioned2022-04-24T22:16:11Z
dc.date.available2022-04-24T22:16:11Z
dc.date.issued2022-02-17
dc.descriptionResearch Areas: Science & Technologypt_PT
dc.description.abstractAnti-CD20 therapies have revolutionized the treatment of B-cell malignancies. Despite these advances, relapsed and refractory disease remains a major treatment challenge. The optimization of CD20-targeted immunotherapies is considered a promising strategy to improve current therapies. However, research has been limited by the scarcity of preclinical models that recapitulate the complex interaction between the immune system and cancers. The addition of the canine lymphoma (cNHL) model in the development of anti-CD20 therapies may provide a clinically relevant approach for the translation of improved immunotherapies. Still, an anti-CD20 therapy for cNHL has not been established stressing the need of a comprehensive target characterization. Herein, we performed an in-depth characterization on canine CD20 mRNA transcript and protein expression in a cNHL biobank and demonstrated a canine CD20 overexpression in B-cell lymphoma samples. Moreover, CD20 gene sequencing analysis identifed six amino acid diferences in patient samples (C77Y, L147F, I159M, L198V, A201T and G273E). Finally, we reported the use of a novel strategy for the generation of anti-CD20 mAbs, with human and canine cross-reactivity, by exploring our rabbit derived singledomain antibody platform. Overall, these results support the rationale of using CD20 as a target for veterinary settings and the development of novel therapeutics and immunodiagnostics.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDias JNR, Almeida A, Andre AS, Aguiar SI, Bule P, Nogueira S, Oliveira SS, Carrapico B, Gil S, Tavares L, Aires-da-Silva F. 2022. Characterization of the canine CD20 as a therapeutic target for comparative passive immunotherapy. Scientific Reports, 12(1):2678. Doi 10.1038/s41598-022-06549-1pt_PT
dc.identifier.doi10.1038/s41598-022-06549-1pt_PT
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.5/24153
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Researchpt_PT
dc.relationDL57/2016/CP1438/CT0002pt_PT
dc.relationDevelopment of novel antibody-drug conjugated molecules for treatment of B-cell malignancies
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-022-06549-1.pdfpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMonoclonal-Antibody Therapypt_PT
dc.subjectB-Cellpt_PT
dc.subjectAnimal-Modelpt_PT
dc.subjectRituximapt_PT
dc.subjectBlymphomapt_PT
dc.subjectExpressionpt_PT
dc.subjectGenept_PT
dc.subjectRepertoirept_PT
dc.subjectCancerpt_PT
dc.subjectIdentificationpt_PT
dc.titleCharacterization of the canine CD20 as a therapeutic target for comparative passive immunotherapypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDevelopment of novel antibody-drug conjugated molecules for treatment of B-cell malignancies
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F90514%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F131468%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.citation.conferencePlaceBerlin, Germanypt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume12(1):2678pt_PT
oaire.fundingStreamSFRH
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
person.familyNameDias
person.familyNameMartinho de Almeida
person.familyNameSantos André
person.familyNameAguiar
person.familyNameBule
person.familyNameCarrapiço
person.familyNameGil Neves
person.familyNameMorgado Tavares
person.familyNameAires da Silva
person.givenNameJoana
person.givenNameAndré
person.givenNameAna Filipa
person.givenNameSandra
person.givenNamePedro
person.givenNameBelmira
person.givenNameSolange
person.givenNameLuis Manuel
person.givenNameFrederico
person.identifier1421801
person.identifierA-3500-2009
person.identifier143307
person.identifier.ciencia-id0813-845C-EFD5
person.identifier.ciencia-id1E14-92CC-B163
person.identifier.ciencia-id511C-998F-DAAD
person.identifier.ciencia-id8E10-C536-8517
person.identifier.ciencia-id171E-F0CF-89F7
person.identifier.ciencia-id2E16-4021-64AB
person.identifier.ciencia-id9011-18C6-482D
person.identifier.ciencia-id4410-6DA0-C74C
person.identifier.orcid0000-0003-1905-1871
person.identifier.orcid0000-0002-7810-3988
person.identifier.orcid0000-0002-1916-6401
person.identifier.orcid0000-0002-3041-383X
person.identifier.orcid0000-0003-2531-9926
person.identifier.orcid0000-0002-1936-5697
person.identifier.orcid0000-0001-9565-6261
person.identifier.orcid0000-0001-8671-6285
person.identifier.orcid0000-0002-3821-419X
person.identifier.scopus-author-id8519566800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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