A carregar...
Publicação
Utilização da citometria de fluxo multiparamétrica na monitorização da resposta fisiológica da levedura Saccharomyces carlsbergensis em presença de ácido acético na produção de bioetanol
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 3.85 MB | Adobe PDF |
Autores
Resumo(s)
With the depletion of fossil fuels, instability of oil market and associated environmental
impacts, there is a renewed interest in biofuels produced from lignocellulosic substrates,
which are a plentiful, cheap and renewable source of biomass for bioethanol production.
The aim of this study was the utilization of multi-parameter flow cytometry in monitoring
the response to stress of Saccharomyces carlsbergensis yeast in the presence of acetic
acid, an inhibitor compound present on lignocellulosic hydrolysates.
From this study it was possible to conclude that the presence of acetic acid affects the
growth (Ki=0,121 L.g-1) and fermentation capability of this yeast, by promoting the increase of
cytoplasmic membrane permeability and depolarization.
The results showed that S. carlsbergensis grows in the presence of acetic acid in
concentrations up to 15 g.L-1, being inhibited at 20 g L-1. However, concentration levels up to
15 g.L-1 still allow the fermentation to occur with global glucose consumption and ethanol
productions at 85% of the assay without inhibitor
Also, in this work, flow cytometry proved to be a useful tool in monitoring this bioprocess,
allowing to detect quickly, accurately and easily, cell responses to the stress caused by the
presence of this inhibitor, and enabling online correction of parameters for improving
industrial fermentation performance.
Descrição
Mestrado em Engenharia de Sistemas Bioenergéticos - Instituto Superior de Agronomia / Faculdade de Ciências. Universidade de Lisboa / Instituto Superior Técnico
Palavras-chave
bioetanol Saccharomyces carlsbergensis acetic acid inhibition multiparameter flow citometry
Contexto Educativo
Citação
Freitas, Cláudia Sofia da Silva - Utilização da citometria de fluxo multiparamétrica na monitorização da resposta fisiológica da levedura Saccharomyces carlsbergensis em presença de ácido acético na produção de bioetanol. Lisboa, 2011