Publication
Activation of adenosine A2A receptors induces TrkB translocation and increases BDNF-mediated phospho-TrkB localization in lipid rafts : implications for neuromodulation
| dc.contributor.author | Assaife-Lopes, Natália | |
| dc.contributor.author | Sousa, Vasco C. | |
| dc.contributor.author | Pereira, Daniela B. | |
| dc.contributor.author | Ribeiro, Joaquim A. | |
| dc.contributor.author | Chao, Moses V. | |
| dc.contributor.author | Sebastião, Ana M | |
| dc.date.accessioned | 2012-05-29T13:04:45Z | |
| dc.date.available | 2012-05-29T13:04:45Z | |
| dc.date.issued | 2010 | |
| dc.description | Copyright © 2010 the authors | eng |
| dc.description.abstract | Brain-derived neurotrophic factor (BDNF) signaling is critical for neuronal development and transmission. Recruitment of TrkB receptors to lipid rafts has been hown to be necessary for the activation of specific signaling pathways and modulation of neurotransmitter release by BDNF. Since TrkB receptors are known to be modulated by adenosine A2A receptor activation, we hypothesized that activation of A2A receptors could influence TrkB receptor localization among different membrane microdomains. We found that adenosine A2A receptor agonists increased the levels of TrkB receptors in the lipid raft fraction of cortical membranes and potentiated BDNF-induced augmentation of phosphorylated TrkB levels in lipid rafts. Blockade of the clathrin-mediated endocytosis with monodansylcadaverine(100µM) did not modify the effects of theA2A receptor agonists but significantly impairedBDNFeffects on TrkB recruitment to lipid rafts. The effect of A2A receptor activation in TrkB localization was mimicked by 5 µM forskolin, an adenylyl cyclase activator. Also, it was blocked by the PKA inhibitors Rp-cAMPs and PKI-(14 –22), and by the Src-family kinase inhibitor PP2. Moreover, removal of endogenous adenosine or disruption of lipid rafts reduced BDNF stimulatory effects on glutamate release from cortical synaptosomes. Lipid raft integrity was also required for the effects of BDNF on hippocampal long-term potentiation at CA1 synapses. Our data demonstrate, for the first time, a BDNF-independent recruitment of TrkB receptors to lipid rafts induced by activation of adenosine A2A receptors, with functional consequences for TrkB phosphorylation and BDNF-induced modulation of neurotransmitter release and hippocampal plasticity. | eng |
| dc.description.sponsorship | Supported by Fundacão para a Ciência e a Tecnologia (SFRH/BD/21374/2005 for N.A.L., SFRH/BD/21359/2005 for V.C.S., and SFRH/BPD/11528/2002 for D.B.P.) and by the European Union [European Cooperation in Science and Technology (COST) COST B30 concerted action, Neural Regeneration and Plasticity (NEREPLAS)]. | por |
| dc.identifier.citation | The Journal of Neuroscience, June 23, 2010 • 30(25):8468–8480 | eng |
| dc.identifier.issn | 0270-6474 | |
| dc.identifier.uri | http://hdl.handle.net/10451/6384 | |
| dc.identifier.uri | DOI:10.1523/JNEUROSCI.5695-09.2010 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Society for Neuroscience | eng |
| dc.subject | Adenosine receptors | eng |
| dc.subject | TrkB | eng |
| dc.subject | Brain-derived neurotrophic factor (BDNF) | eng |
| dc.subject | Lipid rafts | eng |
| dc.subject | Neuromodulation | eng |
| dc.title | Activation of adenosine A2A receptors induces TrkB translocation and increases BDNF-mediated phospho-TrkB localization in lipid rafts : implications for neuromodulation | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 8480 | por |
| oaire.citation.startPage | 8468 | por |
| oaire.citation.title | The Journal of Neuroscience | eng |
| person.familyName | Sebastião | |
| person.givenName | Ana M | |
| person.identifier.ciencia-id | F112-55E8-E37E | |
| person.identifier.orcid | 0000-0001-9030-6115 | |
| person.identifier.scopus-author-id | 7004409879 | |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | 304abd7f-071b-4447-a8a3-4aa5f0547141 | |
| relation.isAuthorOfPublication.latestForDiscovery | 304abd7f-071b-4447-a8a3-4aa5f0547141 |