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Functional analysis of Chestnut and Phytophthora genes involved in plant-pathogen interaction
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Orientador(es)
Resumo(s)
Ink disease, caused by the oomycete Phytophthora cinnamomi Rands, is a form of root rot that affects many woody plants worldwide, including chestnut trees. This pathogen has characteristics, such as long-lasting and chemical-resistant oospores, which make it considerably hard to control. Both the European (Castanea sativa Mill.) and the American [Castanea dentata (Marshall) Borkh.] chestnut species are susceptible to P. cinnamomi, having been historically affected by this oomycete. In contrast, the Asian chestnut species Castanea crenata Sieb. and Zucc. is resistant. The expression of the putative resistance gene Cast_Gnk2-like in this species might be one of the factors contributing to defense. Taking advantage of recently published data regarding the functional validation of Cast_Gnk2-like as a relevant defense factor and P. cinnamomi transcriptome in resistant and susceptible chestnut species throughout the infection process, the goals of this work are: 1) assess Cast_Gnk2-like overexpression from transformed C. sativa embryo clumps; 2) analyze the effect of Cast_Gnk2-like overexpression of transformed C. dentata on P. cinnamomi gene expression during the infection process; 3) analyze P. cinnamomi proteins using in silico tools to complement our understanding of their function and properties. We found that 3 out of 6 different transformed C. sativa lines had high enough significant overexpression and were selected for plant regeneration and further studies. Due to limitations of the study, no conclusions could be taken from the C. dentata inoculation assays, but in silico analysis results revealed many insights regarding the properties of the selected P. cinnamomi proteins.
Descrição
Tese de mestrado, Biologia Molecular e Genética, 2026, Universidade de Lisboa, Faculdade de Ciências
Palavras-chave
Castanea Cast_Gnk2-like host-pathogen interaction oomycete qPCR