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Early infant diagnosis of HIV-1 infection in Luanda, Angola, using a new DNA PCR assay and dried blood spots

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dc.contributor.authorMartin, Francisco
dc.contributor.authorPalladino, Claudia
dc.contributor.authorMateus, Rita
dc.contributor.authorBolzan, Anna
dc.contributor.authorGomes, Perpetua
dc.contributor.authorBrito, José
dc.contributor.authorCarvalho, Ana Patrícia
dc.contributor.authorCardoso, Yolanda
dc.contributor.authorDomingos, Cristovão
dc.contributor.authorClemente, Vanda Sofia Lôa
dc.contributor.authorTaveira, Nuno
dc.date.accessioned2023-08-31T20:01:47Z
dc.date.available2023-08-31T20:01:47Z
dc.date.issued2017
dc.date.updated2023-02-09T14:53:51Z
dc.description.abstractBackground Early diagnosis and treatment reduces HIV-1-related mortality, morbidity and size of viral reservoirs in infants infected perinatally. Commercial molecular tests enable the early diagnosis of infection in infants but the high cost and low sensitivity with dried blood spots (DBS) limit their use in sub-Saharan Africa. Objectives To develop and validate a sensitive and cheap qualitative proviral DNA PCR-based assay for early infant diagnosis (EID) in HIV-1-exposed infants using DBS samples. Study design Chelex-based method was used to extract DNA from DBS samples followed by a nested PCR assay using primers for the HIV-1 integrase gene. Limit of detection (LoD) was determined by Probit regression using limiting dilutions of newly produced recombinant plasmids with the integrase gene of all HIV-1 subtypes and ACH-2 cells. Clinical sensitivity and specificity were evaluated on 100 HIV-1 infected adults; 5 infected infants; 50 healthy volunteers; 139 HIV-1-exposed infants of the Angolan Pediatric HIV Cohort (APEHC) with serology at 18 months of life. Results All subtypes and CRF02_AG were amplified with a LoD of 14 copies. HIV-1 infection in infants was detected at month 1 of life. Sensitivity rate in adults varied with viral load, while diagnostic specificity was 100%. The percentage of HIV-1 MTCT cases between January 2012 and October 2014 was 2.2%. The cost per test was 8-10 USD which is 2- to 4-fold lower in comparison to commercial assays. Conclusions The new PCR assay enables early and accurate EID. The simplicity and low-cost of the assay make it suitable for generalized implementation in Angola and other resource-constrained countries.pt_PT
dc.description.sponsorshipFinancial support was provided by the Fundação para a Ciência e a Tecnologia (FCT) Portugal (PTDC/SAU-EPI/122400/2010), part of the EDCTP2 programme supported by the European Union. Francisco Martin is supported by the Portuguese Fundação para a Ciência e Tecnologia (FCT) (grant number SFRH/BD/87488/2012). Claudia Palladino is supported by the Portuguese Fundação para a Ciência e Tecnologia (FCT) (grant number SFRH/BPD/77448/2011).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMartin F, Palladino C, Mateus R, Bolzan A, Gomes P, Brito J, et al. Early infant diagnosis of HIV-1 infection in Luanda, Angola, using a new DNA PCR assay and dried blood spots. PLOS ONE [Internet]. 17 de julho de 2017;12(7):e0181352. Disponível em: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0181352pt_PT
dc.identifier.doi10.1371/journal.pone.0181352pt_PT
dc.identifier.eid2-s2.0-85024842860
dc.identifier.slugcv-prod-1185525
dc.identifier.urihttp://hdl.handle.net/10451/59113
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPlospt_PT
dc.relationMolecular epidemiology, drug resistance and pathogenesis of HIV and TB in Angola: the Angolan PErinatal HIV Cohort (APEHC)
dc.relationHIV EPIDEMIOLOGY AND PATHOGENESIS IN WOMEN AND CHILDREN IN LUANDA, ANGOLA
dc.relationEPIDEMIOLOGY, PATHOGENESIS AND DETERMINANTS OF HIV-1 TRANSMISSION AND DISEASE PROGRESSION IN THE ANGOLAN PERINATAL HIV COHORT APEHC
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0181352pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleEarly infant diagnosis of HIV-1 infection in Luanda, Angola, using a new DNA PCR assay and dried blood spotspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMolecular epidemiology, drug resistance and pathogenesis of HIV and TB in Angola: the Angolan PErinatal HIV Cohort (APEHC)
oaire.awardTitleHIV EPIDEMIOLOGY AND PATHOGENESIS IN WOMEN AND CHILDREN IN LUANDA, ANGOLA
oaire.awardTitleEPIDEMIOLOGY, PATHOGENESIS AND DETERMINANTS OF HIV-1 TRANSMISSION AND DISEASE PROGRESSION IN THE ANGOLAN PERINATAL HIV COHORT APEHC
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-EPI%2F122400%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F87488%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBPD%2F77448%2F2011/PT
oaire.citation.issue7pt_PT
oaire.citation.startPagee0181352pt_PT
oaire.citation.titlePLoS ONEpt_PT
oaire.citation.volume12pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamOE
oaire.fundingStreamOE
person.familyNameMartin
person.familyNamePalladino
person.familyNameMateus
person.familyNameRodrigues Gomes Cavaco Silva
person.familyNameTaveira
person.givenNameFrancisco
person.givenNameClaudia
person.givenNameRita
person.givenNamePerpétua da Conceição
person.givenNameNuno
person.identifier.ciencia-idCE1B-02AA-5A3C
person.identifier.ciencia-id721D-6851-20D5
person.identifier.ciencia-id7219-B8D3-2942
person.identifier.ciencia-id7111-3383-A926
person.identifier.orcid0000-0002-9599-7805
person.identifier.orcid0000-0002-8148-0928
person.identifier.orcid0000-0002-4883-3760
person.identifier.orcid0000-0003-3271-8255
person.identifier.orcid0000-0003-0176-5585
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid721D-6851-20D5 | Claudia Palladino
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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