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Ecological drivers of Mycobacterium avium subsp. paratuberculosis detection in mongoose (Herpestes ichneumon) using IS900 as proxy

2020-01Journal article Open access
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dc.contributor.authorCunha, Mónica V.
dc.contributor.authorRosalino, L. M.
dc.contributor.authorLeão, Célia
dc.contributor.authorBandeira, Victor
dc.contributor.authorFonseca, Carlos
dc.contributor.authorBotelho, Ana
dc.contributor.authorReis, Ana C.
dc.date.accessioned2021-01-31T13:53:21Z
dc.date.available2021-01-31T13:53:21Z
dc.date.issued2020-01
dc.description.abstractMycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of Johne's disease or paratuberculosis, a chronic infection affecting domestic ruminants worldwide. Despite sporadic reports of MAP occurrence in non-ruminants, information on the risk factors predisposing for infection is still scarce and evidence of transmission paths linking the livestock-wildlife-environment interfaces also remains lacking. In this study, we predicted that environmental, host-related, land use and human driven disturbance factors would modulate carnivore exposure to MAP. To test these hypotheses, we performed a retrospective survey, based on microbiological and molecular methods, in mainland Portugal including five sympatric species from the Herpestidae, Canidae, Viverridae, and Mustelidae families (n = 202) and examined 16 variables as putative predictors of MAP occurrence. Molecular evidence of MAP using IS900 as proxy was demonstrated in 7.43% (95%CI: 4.55-11.9) of surveyed carnivores, the highest proportions being registered for red fox (Vulpes vulpes) (10%; 95%CI: 4.0-23) and Egyptian mongoose (Herpestes ichneumon) (6.0%; 95%CI: 3.2-11). We demonstrate that important species of the Mediterranean carnivore guild, such as stone marten (Martes foina) and common genet (Genetta genetta), may also be exposed to MAP, being this the first time that occurrence in genet is reported. The high proportion of DNA-positive specimens, concurrent with the apparent lack of gastro-enteric lesions and molecular confirmation of IS900 in feces, argue for the presence of subclinical carriers that occasionally shed bacteria, potentially aiding as source of infection to susceptible species and possibly contributing for environmental contamination. Achievement of MAP isolation would prove beyond any doubt that MAP is present in this wildlife population. Ecological modelling results suggested that the probability of MAP infection using IS900 as proxy in mongoose is positively associated with higher altitude and temperature stability, as well as with lower annual rainfall. Density of livestock farms was found not to be a significant predictor, which may indicate that the livestock-wildlife interface is probably not important as an infection route for mongoose.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCunha, M.V., Rosalino, L.M., Leão, C. et al. Ecological drivers of Mycobacterium avium subsp. paratuberculosis detection in mongoose (Herpestes ichneumon) using IS900 as proxy. Sci Rep 10, 860 (2020). https://doi.org/10.1038/s41598-020-57679-3pt_PT
dc.identifier.doi10.1038/s41598-020-57679-3pt_PT
dc.identifier.urihttp://hdl.handle.net/10451/46085
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNaturept_PT
dc.relationPOCI-010145-FEDER-029783pt_PT
dc.relationCentre for Ecology, Evolution and Environmental Changes
dc.relationBiosystems & Integrative Sciences Institute
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAltitudept_PT
dc.subjectEgyptian mongoosept_PT
dc.subjectWild carnivorespt_PT
dc.subjectEnvironmental epidemiologypt_PT
dc.subjectHerpestidaept_PT
dc.subjectMycobacterium avium subsp. paratuberculosispt_PT
dc.subjectParatuberculosispt_PT
dc.subjectEcological factorspt_PT
dc.subjectTemperaturept_PT
dc.subjectMediterranean agroecosystemspt_PT
dc.titleEcological drivers of Mycobacterium avium subsp. paratuberculosis detection in mongoose (Herpestes ichneumon) using IS900 as proxypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Ecology, Evolution and Environmental Changes
oaire.awardTitleBiosystems & Integrative Sciences Institute
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIA%2F00329%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04046%2F2019/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage860pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCunha
person.familyNameRosalino
person.givenNameMonica V.
person.givenNameLuís Miguel
person.identifier348477
person.identifierB-9406-2009
person.identifier.ciencia-id5616-E715-2F31
person.identifier.ciencia-id9D1D-B42A-286C
person.identifier.orcid0000-0003-0401-0276
person.identifier.orcid0000-0003-4186-7332
person.identifier.ridD-5544-2011
person.identifier.scopus-author-id7102711216
person.identifier.scopus-author-id6506527252
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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