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Advisor(s)
Abstract(s)
Drought is a major constraint to plant growth and productivity worldwide and will
aggravate as water availability becomes scarcer. Although elevated air [CO2] might mitigate some of
these effects in plants, the mechanisms underlying the involved responses are poorly understood
in woody economically important crops such as Coffea. This study analyzed transcriptome changes
in Coffea canephora cv. CL153 and C. arabica cv. Icatu exposed to moderate (MWD) or severe water
deficits (SWD) and grown under ambient (aCO2) or elevated (eCO2) air [CO2]. We found that
changes in expression levels and regulatory pathways were barely affected by MWD, while the SWD
condition led to a down-regulation of most differentially expressed genes (DEGs). eCO2 attenuated
the impacts of drought in the transcripts of both genotypes but mostly in Icatu, in agreement with
physiological and metabolic studies. A predominance of protective and reactive oxygen species
(ROS)-scavenging-related genes, directly or indirectly associated with ABA signaling pathways, was
found in Coffea responses, including genes involved in water deprivation and desiccation, such as
protein phosphatases in Icatu, and aspartic proteases and dehydrins in CL153, whose expression
was validated by qRT-PCR. The existence of a complex post-transcriptional regulatory mechanism
appears to occur in Coffea explaining some apparent discrepancies between transcriptomic, proteomic,
and physiological data in these genotypes.
Description
Keywords
ABA signaling coffee functional analysis ROS stress tolerance
Pedagogical Context
Citation
Marques, I.; Fernandes, I.; Paulo, O.S.; Batista, D.; Lidon, F.C.; Partelli, F.; DaMatta, F.M.; Ribeiro-Barros, A.I.; Ramalho, J.C. Overexpression of water-responsive genes promoted by elevated CO2 reduces ROS and enhances drought tolerance in coffea species. Int. J. Mol. Sci. 2023, 24, 3210.
Publisher
MDPI