Logo do repositório
 
Publicação

Multicompartment microreactors prevent excitotoxic dysfunctions in rat primary cortical neurons

2020Artigo científico Acesso restrito
Ver registo simples
dc.contributor.authorArmada-Moreira, Adam
dc.contributor.authorCoelho, Joana E
dc.contributor.authorLopes, Luisa V.
dc.contributor.authorSebastião, Ana M
dc.contributor.authorStädler, Brigitte
dc.contributor.authorVaz, Sandra H.
dc.date.accessioned2022-12-13T14:19:00Z
dc.date.available2022-12-13T14:19:00Z
dc.date.issued2020
dc.description© 2020 Wiley-VCH GmbHpt_PT
dc.description.abstractExcitotoxicity is a cellular phenomenon that comprises the consequences of toxic actions of excitatory neurotransmitters, such as glutamate. This process is usually related to overproduction of reactive oxygen species (ROS) and ammonia (NH4+ ) toxicity. Platinum nanoparticle (Pt-NP)-based microreactors able to degrade hydrogen peroxide (H2 O2 ) and NH4+ , are previously described as a novel therapeutical approach against excitotoxicity, conferring protection to neuroblasts. Now, it is demonstrated that these microreactors are compatible with rat primary cortical neurons, show high levels of neuronal membrane interaction, and are able to improve cell survival and neuronal activity when neurons are exposed to H2 O2 or NH4+ . Additionally, more complex microreactors are assembled, including enzyme-loaded liposomes containing glutamate dehydrogenase and glutathione reductase, in addition to Pt-NP. The in vitro activity of these microreactors is characterized and they are compared to the Pt-NP-based microreactors in terms of biological activity, concluding that they enhance cell viability similarly or more extensively than the latter. Extracellular electrophysiological recordings demonstrate that these microreactors rescue neuronal functionality lost upon incubation with H2 O2 or NH4+ . This study provides more evidence for the potential application of these microreactors in a biomedical context with more complex cellular environments.pt_PT
dc.description.sponsorshipThis work was supported by Fundação para a Ciência e Tecnologia (FCT), Portugal (PTDC/BTM-SAL/32147/2017, PD/BD/114278/2016, IMM/BI/2-2020) and the Aarhus University Research Foundation, Denmark.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAdv Biosyst. 2020 Oct;4(10):e2000139pt_PT
dc.identifier.doi10.1002/adbi.202000139pt_PT
dc.identifier.eissn2701-0198
dc.identifier.urihttp://hdl.handle.net/10451/55381
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationAstrosomes: Artificial Astrocytes as a Novel Approach to Regulate Neuronal Communication
dc.relationAstrosomes: a novel approach for the regulation of neuronal communication
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/journal/27010198pt_PT
dc.subjectEncapsulated catalysispt_PT
dc.subjectExcitotoxicitypt_PT
dc.subjectMicroreactorspt_PT
dc.subjectPlatinum nanoparticlespt_PT
dc.subjectRat cortical primary neuronspt_PT
dc.titleMulticompartment microreactors prevent excitotoxic dysfunctions in rat primary cortical neuronspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberPTDC/BTM-SAL/32147/2017
oaire.awardNumberPD/BD/114278/2016
oaire.awardTitleAstrosomes: Artificial Astrocytes as a Novel Approach to Regulate Neuronal Communication
oaire.awardTitleAstrosomes: a novel approach for the regulation of neuronal communication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBTM-SAL%2F32147%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//PD%2FBD%2F114278%2F2016/PT
oaire.citation.issue10pt_PT
oaire.citation.titleAdvanced Biosystemspt_PT
oaire.citation.volume4pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameArmada-Moreira
person.familyNameCoelho
person.familyNameLopes
person.familyNameSebastião
person.familyNameHenriques Vaz
person.givenNameAdam
person.givenNameJoana
person.givenNameLuisa
person.givenNameAna M
person.givenNameSandra Cristina
person.identifier695915
person.identifier149432
person.identifier.ciencia-id5513-2AB8-C621
person.identifier.ciencia-idB310-839B-564D
person.identifier.ciencia-id4817-7194-C024
person.identifier.ciencia-idF112-55E8-E37E
person.identifier.ciencia-id0E1C-952D-61BE
person.identifier.orcid0000-0002-1598-5784
person.identifier.orcid0000-0002-3964-6197
person.identifier.orcid0000-0001-8367-3005
person.identifier.orcid0000-0001-9030-6115
person.identifier.orcid0000-0003-4258-9397
person.identifier.ridS-7608-2017
person.identifier.scopus-author-id7101840699
person.identifier.scopus-author-id7102509159
person.identifier.scopus-author-id7004409879
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication05481919-e17c-439f-946b-3be55e653c0e
relation.isAuthorOfPublication1301afd3-8056-4d54-983c-0bec935b2d75
relation.isAuthorOfPublicationdfc15976-b186-45d1-be1c-4592b336edb1
relation.isAuthorOfPublication304abd7f-071b-4447-a8a3-4aa5f0547141
relation.isAuthorOfPublicationb282e0fa-69eb-4079-8752-b7dd914f7745
relation.isAuthorOfPublication.latestForDiscovery304abd7f-071b-4447-a8a3-4aa5f0547141
relation.isProjectOfPublication1945faf0-0fa5-48d5-90b4-00ae1f0fef66
relation.isProjectOfPublicatione9988f13-7c93-4a05-a535-4462993a34e9
relation.isProjectOfPublication.latestForDiscoverye9988f13-7c93-4a05-a535-4462993a34e9

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
Multicompartment_microreactors.pdf
Tamanho:
1.2 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.2 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

IMM - Artigos em Revistas Internacionais
FM - Artigos em Revistas Internacionais