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Spontaneous CO release from RuII(CO)2-protein complexes in aqueous solution, cells, and mice

2015Artigo científico Acesso aberto
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dc.contributor.authorChaves-Ferreira, Miguel
dc.contributor.authorAlbuquerque, Inês S.
dc.contributor.authorMatak-Vinkovic, Dijana
dc.contributor.authorCoelho, Ana C.
dc.contributor.authorCarvalho, Sandra M.
dc.contributor.authorSaraiva, Lígia M.
dc.contributor.authorRomão, Carlos C.
dc.contributor.authorBernardes, Gonçalo J. L.
dc.date.accessioned2022-03-15T16:33:25Z
dc.date.available2022-03-15T16:33:25Z
dc.date.issued2015
dc.description© 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.pt_PT
dc.description.abstractWe demonstrate that Ru(II)(CO)2-protein complexes, formed by the reaction of the hydrolytic decomposition products of [fac-RuCl(κ(2)-H2NCH2CO2)(CO)3] (CORM-3) with histidine residues exposed on the surface of proteins, spontaneously release CO in aqueous solution, cells, and mice. CO release was detected by mass spectrometry (MS) and confocal microscopy using a CO-responsive turn-on fluorescent probe. These findings support our hypothesis that plasma proteins act as CO carriers after in vivo administration of CORM-3. CO released from a synthetic bovine serum albumin (BSA)-Ru(II)(CO)2 complex leads to downregulation of the cytokines interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α in cancer cells. Finally, administration of BSA-Ru(II)(CO)2 in mice bearing a colon carcinoma tumor results in enhanced CO accumulation at the tumor. Our data suggest the use of Ru(II)(CO)2-protein complexes as viable alternatives for the safe and spatially controlled delivery of therapeutic CO in vivo.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAngew. Chem. Int. Ed. 2015, 54, 1172-1175pt_PT
dc.identifier.doi10.1002/anie.201409344pt_PT
dc.identifier.eissn1521-3773
dc.identifier.issn1433-7851
dc.identifier.urihttp://hdl.handle.net/10451/51756
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/journal/15213773pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCORMpt_PT
dc.subjectAlbuminpt_PT
dc.subjectCarbon monoxidept_PT
dc.subjectMetalloproteinspt_PT
dc.subjectProdrugspt_PT
dc.titleSpontaneous CO release from RuII(CO)2-protein complexes in aqueous solution, cells, and micept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1175pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage1172pt_PT
oaire.citation.titleAngewandte Chemie International Editionpt_PT
oaire.citation.volume54pt_PT
person.familyNameCorreia Botelho Chaves Ferreira
person.familyNameS. Albuquerque
person.familyNameBernardes
person.givenNameMiguel
person.givenNameInês
person.givenNameGonçalo
person.identifier.ciencia-id4B11-39A0-F921
person.identifier.ciencia-idAA13-20AA-F50C
person.identifier.orcid0000-0002-5375-3562
person.identifier.orcid0000-0002-1108-0961
person.identifier.orcid0000-0001-6594-8917
person.identifier.ridP-5325-2016
person.identifier.scopus-author-id14046757500
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication9f2a595e-3548-40d7-92e4-114b73cba343
relation.isAuthorOfPublication37a8c939-e874-41d7-86bd-9d6d349467e4
relation.isAuthorOfPublicationd1a48067-77b1-4413-b1c7-602fb18c62c0
relation.isAuthorOfPublication.latestForDiscovery9f2a595e-3548-40d7-92e4-114b73cba343

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