Publication
Efficient cellular delivery of β-Galactosidase mediated by NrTPs, a new family of cell-penetrating peptides
| dc.contributor.author | Rodrigues, Margarida | |
| dc.contributor.author | de la Torre, Beatriz G. | |
| dc.contributor.author | Rádis-Baptista, Gandhi | |
| dc.contributor.author | Santos, Nuno C. | |
| dc.contributor.author | Andreu, David | |
| dc.date.accessioned | 2012-07-23T13:10:37Z | |
| dc.date.available | 2012-07-23T13:10:37Z | |
| dc.date.issued | 2011 | |
| dc.description | © 2011 American Chemical Society | eng |
| dc.description.abstract | Nucleolar targeting peptides (NrTPs), a recently developed family of cell-penetrating peptides, have been shown to be very efficient in entering cells and accumulating in their nucleoli. In this work, we have used conjugates of NrTP6 (YKQSHKKGGKKGSG) covalently linked to β-galactosidase in order to demonstrate the capacity of NrTP for intracellular delivery of large molecules. NrTP6/β-galactosidase conjugates, prepared by maleimide-based chemistry, were stable and enzymatically active on the standard 4-methylumbelliferyl β-D-galactopyranoside substrate. Their translocation into HeLa cells, monitored by β-galactosidase activity as a readout of the uptake, showed efficient cellular entry and thus demonstrated the potential of NrTPs for intracellular delivery of large-size cargos with preservation of biological activity. | eng |
| dc.description.sponsorship | This work was funded by the Portuguese Ministry of Science, Technology and Higher Education (Fundação para a Ciência e a Tecnologia, FCT-MCTES; including M.R. PhD grant SFRH/BD/37432/2007), the Spanish Ministry of Science and Innovation (MICINN, grant BIO2008-04487-CO3 to D.A.), the European Union 7th Framework Program (IRSES project MEMPEPACROSS) and the European Biophysical Societies Association (EBSA | eng |
| dc.identifier.citation | Bioconjugate Chem. 2011, 22, 2339−2344 | por |
| dc.identifier.issn | 1043-1802 | |
| dc.identifier.uri | http://dx.doi.org/10.1021/bc200421z | |
| dc.identifier.uri | http://hdl.handle.net/10451/6747 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | American Chemical Society | eng |
| dc.title | Efficient cellular delivery of β-Galactosidase mediated by NrTPs, a new family of cell-penetrating peptides | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 2344 | por |
| oaire.citation.startPage | 2339 | por |
| oaire.citation.title | Bioconjugate Chemistry | por |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |