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A new clonal propagation protocol develops quality root systems in chestnut
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Orientador(es)
Resumo(s)
There is, at the present time, a great demand for chestnut rootstocks with improved resistance
to Phytophthora cinnamomi Rands in the nurseries. New genotypes are emerging from European
chestnut breeding programs and the production of thriving plants to restore old orchards with low
yields due to a high incidence of diseases, namely root rot, is necessary. Micropropagation is a useful
technique for clonal propagation. Nevertheless, in vitro culture propagation is genotype-dependent.
Consequently, the existing protocols may demonstrate poor reproducibility and low e cacy.
Thus, the need to contribute to the development of new micropropagation protocols suitable
for large production of emerging genotypes. As a contribution to fill this gap, a three-step protocol
was developed by using new combinations of Murashige & Skoog, Woody Plant, and adapted
modified Melin-Norkrans media in di erent stages of the propagation process. About 90% of shoots
were rooted, and after three months of acclimatization, 85% of these plants survived and were capable
of continuous growth in the field. Currently, this protocol is being used in the production of several
hybrid genotypes (with improved resistance to P. cinnamomi), selected from our ongoing breeding
program and also in Castanea sativa Mill. and Castanea crenata Siebold and Zucc. species
Descrição
Technical Note
Palavras-chave
axillary shoot proliferation Castanea hybrids rooting rootstocks tissue culture
Contexto Educativo
Citação
Forests 2020, 11, 826
Editora
MDPI