Logo do repositório
 
Publicação

The EuroFlow PID orientation tube for flow cytometric diagnostic screening of primary immunodeficiencies of the lymphoid system

2019Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorvan der Burg, Mirjam
dc.contributor.authorKalina, Tomas
dc.contributor.authorPerez-Andres, Martin
dc.contributor.authorVlkova, Marcela
dc.contributor.authorLopez-Granados, Eduardo
dc.contributor.authorBlanco, Elena
dc.contributor.authorBonroy, Carolien
dc.contributor.authorSousa, Ana E.
dc.contributor.authorKienzler, Anne-Kathrin
dc.contributor.authorWentink, Marjolein
dc.contributor.authorMejstríková, Ester
dc.contributor.authorŠinkorova, Vendula
dc.contributor.authorStuchly, Jan
dc.contributor.authorvan Zelm, Menno C.
dc.contributor.authorOrfao, Alberto
dc.contributor.authorvan Dongen, Jacques J. M.
dc.date.accessioned2021-12-10T15:31:00Z
dc.date.available2021-12-10T15:31:00Z
dc.date.issued2019
dc.descriptionCopyright © 2019 van der Burg, Kalina, Perez-Andres, Vlkova, Lopez-Granados, Blanco, Bonroy, Sousa, Kienzler, Wentink, Mejstríková, Šinkorova, Stuchly, van Zelm, Orfao and van Dongen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.pt_PT
dc.description.abstractIn the rapidly evolving field of primary immunodeficiencies (PID), the EuroFlow consortium decided to develop a PID orientation and screening tube that facilitates fast, standardized, and validated immunophenotypic diagnosis of lymphoid PID, and allows full exchange of data between centers. Our aim was to develop a tool that would be universal for all lymphoid PIDs and offer high sensitivity to identify a lymphoid PID (without a need for specificity to diagnose particular PID) and to guide and prioritize further diagnostic modalities and clinical management. The tube composition has been defined in a stepwise manner through several cycles of design-testing-evaluation-redesign in a multicenter setting. Equally important appeared to be the standardized pre-analytical procedures (sample preparation and instrument setup), analytical procedures (immunostaining and data acquisition), the software analysis (a multidimensional view based on a reference database in Infinicyt software), and data interpretation. This standardized EuroFlow concept has been tested on 250 healthy controls and 99 PID patients with defined genetic defects. In addition, an application of new EuroFlow software tools with multidimensional pattern recognition was designed with inclusion of maturation pathways in multidimensional patterns (APS plots). The major advantage of the EuroFlow approach is that data can be fully exchanged between different laboratories in any country of the world, which is especially of interest for the PID field, with generally low numbers of cases per center.pt_PT
dc.description.sponsorshipThe coordination and innovation processes of this study were supported by the EuroFlow Consortium (Chairmen: MvdB and AO). MvZ is supported by Senior Research Fellowship GNT1117687 from the Australian National Health and Medical Research Council. TK and EM were supported by projects 15-28541A from Ministry of Health, LO1604 from Ministry of Education, Youth and Sports and GBP302/12/G101 from Grant Agency of the Czech Republic. MP-A, EB, and AO were supported by a grant from the Junta de Castilla y León (Fondo Social Europeo, ORDEN EDU/346/2013, Valladolid, Spain) and the CB16/12/00400 grant (CIBER/ONC, Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, - Madrid, Spain- and FONDOS FEDER), the FIS PI12/00905-FEDER grant (Fondo de Investigación Sanitaria of Instituto de Salud Carlos III, Madrid, Spain) and AP119882013 grant (Fundación Mutua Madrileña, Madrid, Spain). Publishing costs for this article were covered by the International Union of Immunological Societies (IUIS).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFront Immunol. 2019 Mar 4;10:246pt_PT
dc.identifier.doi10.3389/fimmu.2019.00246pt_PT
dc.identifier.eissn1664-3224
dc.identifier.urihttp://hdl.handle.net/10451/50342
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontierspt_PT
dc.relation.publisherversionhttps://www.frontiersin.org/journals/immunology#pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEuroFlowpt_PT
dc.subjectAutomated gating strategypt_PT
dc.subjectFlow cytometric immunophenotypingpt_PT
dc.subjectPrimary immunodeficienciespt_PT
dc.subjectStandardizationpt_PT
dc.titleThe EuroFlow PID orientation tube for flow cytometric diagnostic screening of primary immunodeficiencies of the lymphoid systempt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleFrontiers in Immunologypt_PT
oaire.citation.volume10pt_PT
person.familyNameSousa
person.givenNameAna E.
person.identifier61187
person.identifier.ciencia-idD015-D3A2-992D
person.identifier.orcid0000-0002-4618-9799
person.identifier.ridL-2642-2014
person.identifier.scopus-author-id7202484563
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication932aed1d-ffa8-494d-b958-43289d2b9cec
relation.isAuthorOfPublication.latestForDiscovery932aed1d-ffa8-494d-b958-43289d2b9cec

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
EuroFlow_PID.pdf
Tamanho:
12.61 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

IMM - Artigos em Revistas Internacionais
FM - Artigos em Revistas Internacionais