Publicação
A 96-wells fluidic system for high-throughput screenings under laminar high wall shear stress conditions
| dc.contributor.author | Fonseca, Catarina | |
| dc.contributor.author | Silvério, Vânia | |
| dc.contributor.author | Barata, David | |
| dc.contributor.author | Giese, Wolfgang | |
| dc.contributor.author | Gerhardt, Holger | |
| dc.contributor.author | Cardoso, Susana | |
| dc.contributor.author | Franco, Claudio | |
| dc.date.accessioned | 2023-09-25T14:12:02Z | |
| dc.date.available | 2023-09-25T14:12:02Z | |
| dc.date.issued | 2023 | |
| dc.description | © The Author(s) 2023. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | pt_PT |
| dc.description.abstract | The ability of endothelial cells to respond to blood flow is fundamental for the correct formation and maintenance of a functional and hierarchically organized vascular network. Defective flow responses, in particular related to high flow conditions, have been associated with atherosclerosis, stroke, arteriovenous malformations, and neurodegenerative diseases. Yet, the molecular mechanisms involved in high flow response are still poorly understood. Here, we described the development and validation of a 96-wells fluidic system, with interchangeable cell culture and fluidics, to perform high-throughput screenings under laminar high-flow conditions. We demonstrated that endothelial cells in our newly developed 96-wells fluidic system respond to fluid flow-induced shear stress by aligning along the flow direction and increasing the levels of KLF2 and KLF4. We further demonstrate that our 96-wells fluidic system allows for efficient gene knock-down compatible with automated liquid handling for high-throughput screening platforms. Overall, we propose that this modular 96-well fluidic system is an excellent platform to perform genome-wide and/or drug screenings to identify the molecular mechanisms involved in the responses of endothelial cells to high wall shear stress. | pt_PT |
| dc.description.sponsorship | C.G.F. was supported by a PhD fellowship from the doctoral program Bioengineering: Cellular Therapies and Regenerative Medicine funded by Fundação para a Ciência e Tecnologia (PD/BD/128375/2017). This work was supported by the European Research Council (679368); European Commission (801423); Fondation LeDucq (17CVD03); Fundação para a Ciência e Tecnologia (PTDC/BIA-CEL/32180/2017; CEECIND/04251/2017; UID/05367/2020; FPJ001377 - PTDC/MED-ANM/7695/2020; FPJ001461 - EXPL/MED-ANM/1616/2021; PTDC-FISPLA/31055/2017; and LA/P/0140/2020). | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Microsyst Nanoeng. 2023 Sep 15;9:114. | pt_PT |
| dc.identifier.doi | 10.1038/s41378-023-00589-x | pt_PT |
| dc.identifier.eissn | 2055-7434 | |
| dc.identifier.issn | 2096-1030 | |
| dc.identifier.uri | http://hdl.handle.net/10451/59457 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Springer Nature | pt_PT |
| dc.relation | UID/05367/2020 | pt_PT |
| dc.relation | Biomechanics of vascular remodelling | |
| dc.relation | PRINCIPLES OF AXIAL POLARITY-DRIVEN VASCULAR PATTERNING | |
| dc.relation | Building a 3D innervated and irrigated muscle on a chip. | |
| dc.relation | Identify the mechanisms of endothelial tip cell invasive behavior in order to inhibit physiological and pathological sprouting angiogenesis | |
| dc.relation | Not Available | |
| dc.relation | LA/P/0140/2020 | pt_PT |
| dc.relation.publisherversion | https://www.nature.com/micronano/ | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Materials science | pt_PT |
| dc.subject | Nanoscale devices | pt_PT |
| dc.title | A 96-wells fluidic system for high-throughput screenings under laminar high wall shear stress conditions | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Biomechanics of vascular remodelling | |
| oaire.awardTitle | PRINCIPLES OF AXIAL POLARITY-DRIVEN VASCULAR PATTERNING | |
| oaire.awardTitle | Building a 3D innervated and irrigated muscle on a chip. | |
| oaire.awardTitle | Identify the mechanisms of endothelial tip cell invasive behavior in order to inhibit physiological and pathological sprouting angiogenesis | |
| oaire.awardTitle | Not Available | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//PD%2FBD%2F128375%2F2017/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/EC/H2020/679368/EU | |
| oaire.awardURI | info:eu-repo/grantAgreement/EC/H2020/801423/EU | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-CEL%2F32180%2F2017/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F04251%2F2017%2FCP1396%2FCT0010/PT | |
| oaire.citation.issue | 1 | pt_PT |
| oaire.citation.title | Microsystems & Nanoengineering | pt_PT |
| oaire.citation.volume | 9 | pt_PT |
| oaire.fundingStream | H2020 | |
| oaire.fundingStream | H2020 | |
| oaire.fundingStream | 3599-PPCDT | |
| oaire.fundingStream | CEEC IND 2017 | |
| person.familyName | Fonseca | |
| person.familyName | Barata | |
| person.familyName | Franco | |
| person.givenName | Catarina | |
| person.givenName | David | |
| person.givenName | Claudio | |
| person.identifier | D-8117-2015 | |
| person.identifier.ciencia-id | CB1D-8BC4-4226 | |
| person.identifier.ciencia-id | 5012-3670-64CD | |
| person.identifier.ciencia-id | F012-B7D6-AE72 | |
| person.identifier.orcid | 0000-0002-1978-0612 | |
| person.identifier.orcid | 0000-0002-8753-7403 | |
| person.identifier.orcid | 0000-0002-2861-3883 | |
| person.identifier.scopus-author-id | 24280736600 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100008530 | |
| project.funder.identifier | http://doi.org/10.13039/501100008530 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | European Commission | |
| project.funder.name | European Commission | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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