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Mammalian NET-Seq reveals genome-wide nascent transcription coupled to RNA processing

2015Artigo científico Acesso aberto
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dc.contributor.authorNojima, Takayuki
dc.contributor.authorGomes, Tomás
dc.contributor.authorGrosso, Ana Rita
dc.contributor.authorKimura, Hiroshi
dc.contributor.authorDye, Michael J.
dc.contributor.authorDhir, Somdutta
dc.contributor.authorCarmo-Fonseca, Maria
dc.contributor.authorProudfoot, Nicholas J.
dc.date.accessioned2022-02-01T15:19:38Z
dc.date.available2022-02-01T15:19:38Z
dc.date.issued2015
dc.description© Copyright © 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)pt_PT
dc.description.abstractTranscription is a highly dynamic process. Consequently, we have developed native elongating transcript sequencing technology for mammalian chromatin (mNET-seq), which generates single-nucleotide resolution, nascent transcription profiles. Nascent RNA was detected in the active site of RNA polymerase II (Pol II) along with associated RNA processing intermediates. In particular, we detected 5'splice site cleavage by the spliceosome, showing that cleaved upstream exon transcripts are associated with Pol II CTD phosphorylated on the serine 5 position (S5P), which is accumulated over downstream exons. Also, depletion of termination factors substantially reduces Pol II pausing at gene ends, leading to termination defects. Notably, termination factors play an additional promoter role by restricting non-productive RNA synthesis in a Pol II CTD S2P-specific manner. Our results suggest that CTD phosphorylation patterns established for yeast transcription are significantly different in mammals. Taken together, mNET-seq provides dynamic and detailed snapshots of the complex events underlying transcription in mammals.pt_PT
dc.description.sponsorshipThis work was supported by funding to N.J.P. (Wellcome Trust Programme [091805/Z/10/Z] and ERC Advanced [339270] Grants) and to M.C.-F. (Fundação Ciência e Tecnologia, Portugal).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCell 161, 526–540, April 23, 2015pt_PT
dc.identifier.doi10.1016/j.cell.2015.03.027pt_PT
dc.identifier.eissn1097-4172
dc.identifier.issn0092-8674
dc.identifier.urihttp://hdl.handle.net/10451/51070
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPolicing mammalian transcriptomes: regulation of long ncRNA synthesis by transcriptional termination, gene loops and R-loops.
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/cellpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.titleMammalian NET-Seq reveals genome-wide nascent transcription coupled to RNA processingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePolicing mammalian transcriptomes: regulation of long ncRNA synthesis by transcriptional termination, gene loops and R-loops.
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/339270/EU
oaire.citation.endPage540pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage526pt_PT
oaire.citation.titleCellpt_PT
oaire.citation.volume161pt_PT
oaire.fundingStreamFP7
person.familyNameGomes
person.familyNameGrosso
person.familyNameCarmo-Fonseca
person.givenNameTomás
person.givenNameAna Rita
person.givenNameMaria
person.identifierI-6656-2013
person.identifier.ciencia-id3E11-841C-AE1B
person.identifier.ciencia-id3D18-571E-133A
person.identifier.ciencia-idB31F-0435-0753
person.identifier.orcid0000-0003-1333-0191
person.identifier.orcid0000-0001-6974-4209
person.identifier.orcid0000-0002-3402-7143
person.identifier.scopus-author-id26639262500
person.identifier.scopus-author-id7007128195
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication607e3b4e-024c-4fae-83fe-e0ed9c3f66a8
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