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A mathematical model of fibrinogen-mediated erythrocyte–erythrocyte adhesion

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dc.contributor.authorLopes, Catarina S.
dc.contributor.authorCurty, Juliana
dc.contributor.authorCarvalho, Filomena Almeida
dc.contributor.authorHernández-Machado, A.
dc.contributor.authorKinoshita, Koji
dc.contributor.authorSantos, Nuno C.
dc.contributor.authorTravasso, Rui D. M.
dc.date.accessioned2023-02-28T12:04:17Z
dc.date.available2023-02-28T12:04:17Z
dc.date.issued2023
dc.description© The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.pt_PT
dc.description.abstractErythrocytes are deformable cells that undergo progressive biophysical and biochemical changes affecting the normal blood flow. Fibrinogen, one of the most abundant plasma proteins, is a primary determinant for changes in haemorheological properties, and a major independent risk factor for cardiovascular diseases. In this study, the adhesion between human erythrocytes is measured by atomic force microscopy (AFM) and its effect observed by micropipette aspiration technique, in the absence and presence of fibrinogen. These experimental data are then used in the development of a mathematical model to examine the biomedical relevant interaction between two erythrocytes. Our designed mathematical model is able to explore the erythrocyte-erythrocyte adhesion forces and changes in erythrocyte morphology. AFM erythrocyte-erythrocyte adhesion data show that the work and detachment force necessary to overcome the adhesion between two erythrocytes increase in the presence of fibrinogen. The changes in erythrocyte morphology, the strong cell-cell adhesion and the slow separation of the two cells are successfully followed in the mathematical simulation. Erythrocyte-erythrocyte adhesion forces and energies are quantified and matched with experimental data. The changes observed on erythrocyte-erythrocyte interactions may give important insights about the pathophysiological relevance of fibrinogen and erythrocyte aggregation in hindering microcirculatory blood flow.pt_PT
dc.description.sponsorshipThis work was supported by Fundação para a Ciência e a Tecnologia – Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal) projects PTDC/EMD-TLM/7289/2020 (all authors), PTDC/BBB-BMD/6307/2014 and UID/BIM/50005/2019 (CSL, FAC, NCS), through Fundos do Orçamento de Estado. JC and RT also thank the support of FEDER funds through the Operational Programme Competitiveness Factors – COMPETE and Fundação para a Ciência e a Tecnologia through the strategic projects UIDB/04564/2020 and UIDP/04564/2020. CSL also acknowledges FCT-MCTES fellowships PD/BD/135045/2017 and COVID/BD/151823/2021, General Programme of the European Molecular Biology Conference – EMBO Short-Term Fellowship 8350, and an EBSA Bursary for a working visit to the University of Southern Denmark to perform the micropipette aspiration technique experiments, as well as Erasmus+ mobility – ERASMUS SMT Student Mobility for Traineeships. AH-M acknowledges support from Ministerio de Ciencia e Innovación (Spain) under project PID2019-106063GB-100 and AGAUR (Generalitat de Catalunya) under project 2017 SGR-1061.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCommun Biol. 2023 Feb 17;6(1):192pt_PT
dc.identifier.doi10.1038/s42003-023-04560-4pt_PT
dc.identifier.eissn2399-3642
dc.identifier.urihttp://hdl.handle.net/10451/56478
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.relationMolecular interactions of circulating fibrinogen as a risk factor for cardiovascular and cerebrovascular pathologies
dc.relationInstituto de Medicina Molecular
dc.relationCenter for Physics of the University of Coimbra
dc.relationCenter for Physics of the University of Coimbra
dc.relationUsing AFM to study erythrocytes biophysical properties on cardiovascular diseases and amyotrophic lateral sclerosis
dc.relationUsing AFM to study erythrocytes biophysical properties on cardiovascular diseases and amyotrophic lateral sclerosis
dc.relation.publisherversionhttps://www.nature.com/commsbio/pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleA mathematical model of fibrinogen-mediated erythrocyte–erythrocyte adhesionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberPTDC/EMD-TLM/7289/2020
oaire.awardNumberPTDC/BBB-BMD/6307/2014
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oaire.awardNumberUIDP/04564/2020
oaire.awardNumberPD/BD/135045/2017
oaire.awardNumberCOVID/BD/151823/2021
oaire.awardTitleMolecular interactions of circulating fibrinogen as a risk factor for cardiovascular and cerebrovascular pathologies
oaire.awardTitleInstituto de Medicina Molecular
oaire.awardTitleCenter for Physics of the University of Coimbra
oaire.awardTitleCenter for Physics of the University of Coimbra
oaire.awardTitleUsing AFM to study erythrocytes biophysical properties on cardiovascular diseases and amyotrophic lateral sclerosis
oaire.awardTitleUsing AFM to study erythrocytes biophysical properties on cardiovascular diseases and amyotrophic lateral sclerosis
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT//COVID%2FBD%2F151823%2F2021/PT
oaire.citation.issue1pt_PT
oaire.citation.titleCommunications Biologypt_PT
oaire.citation.volume6pt_PT
oaire.fundingStream3599-PPCDT
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person.familyNamede Sousa Lopes
person.familyNameCarvalho
person.familyNameSantos
person.givenNameCatarina Isabel
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person.givenNameNuno
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rcaap.typearticlept_PT
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