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Avaliação da atividade anti-inflamatória de um extrato de framboesa na Artrite Reumatóide
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Resumo(s)
A framboesa, vulgarmente classificada como um fruto vermelho possui um elevado teor de compostos fenólicos aos quais têm sido associados benefícios para a saúde, relacionados com a sua atividade antioxidante e anti-inflamatória.
Com o principal objetivo de avaliar a atividade anti-inflamatória de um extrato de framboesa em modelo animal de artrite reumatóide, preparou-se um extrato de framboesa recorrendo a uma variedade desconhecida da região Sul de Portugal.
O extrato produzido foi caracterizado quimicamente por ensaios espetrofotométricos e cromatográficos (HPLC-DAD-ED e HPLC-DAD-ESI-MS/MS).
A atividade antioxidante do extrato de framboesa foi avaliada em ensaios ORAC (Oxygen Radical Absorbance Capacity), HORAC (Hydroxyl Radical Antioxidant Capacity) e CAA (Cellular Antioxidant Activity), tendo-se obtido valores de 1277 ± 38 μmol de
equivalentes de Trolox/ 100 g, 529 ± 33 μmol de equivalentes de ácido cafeico/ 100 g e 133 μmol de equivalentes de quercetina equivalentes/ 100 g, respetivamente.
Para o estudo da inflamação aguda, num modelo de edema da pata induzido pela carragenina, administrou-se uma dose única de extrato de framboesa (15 mg de fenóis totais/ kg de rato), usando a via oral e a intraperitoneal. Após análise estatística dos resultados relativos à percentagem de aumento de volume de pata por ANOVA e teste de Bonferroni concluiu-se que a administração de extrato de framboesa via p.o não inibiu o aumento do volume da pata (P<0,001 vs Controlo) enquanto que a administração via i.p causou um efeito de inibição da inflamação (P <0,001 vs Carragenina). No ensaio da inflamação crónica com duração de 35 dias, num modelo de artrite reumatóide (AR) induzida por colagénio tipo II (CII), administrou-se o extrato de framboesa (15 mg de fenóis totais/ kg de rato) por via oral ou intraperitoneal, do 23º ao 35º dias de ensaio).
Para além da redução do edema nas patas dos animais tratados, a análise radiográfica das patas destes animais revelou que a osteoesclerose das superfícies articulares e as áreas erosivas subcondrais das articulações das patas posteriores eram menos evidentes nos ratos aos quais foi administrado extrato de framboesa por via oral ou por via intraperitoneal, o que sugere a ação anti-inflamatória do extrato de framboesa quando administrado continuadamente.
Raspberries are classified as a berry and have a high content of phenolic compounds. Those compounds have been associated with health benefits due to their antioxidant and antiinflammatory effects. The main objective was to evaluate the anti-inflammatory effect of a raspberry extract in the animal model Arthritis Rheumatoid. It was prepared from an extract of an unknown raspberry variety from the south of Portugal. The extract was chemically analysed by spectrophotometric and chromatographic assays (HPLC-DAD-ED e HPLC-DAD-ESI-MS/MS). The antioxidant activity of the raspberry extract was evaluated by ORAC (Oxygen Radical Absorbance Capacity), HORAC (Hydroxyl Radical Antioxidant Capacity) and CAA (Cellular Antioxidant Activity) assays. It has obtained values of 1277 ± 38 μmol of Trolox equivalents per 100 g of fresh fruit, 529 ± 33 μmol of cafeic acid equivalents/ 100 g and 133 μmol of quercetin equivalents/ 100 g, respectively. For the acute inflammation study, in the carrageenaan induced-paw edema model, a dose of the raspberry extract (15mg of total phenols per Kg of animal weight) was administred, orally and intra-peritoneally. After the statistical analysis (ANOVA and Bonferroni’s test) of the paw’s volume increase, it was demonstrated that the oral extract administration did not inhibit the paw’s volume increase (P<0,001 vs Control) but the intra-peritoneal extract administration did inhibit the inflammatory effect (P <0,001 vs carrageenaan). The duration of the chronic inflammation assay was 35 days. It was an animal model of collagen-induced arthritis. The raspberry extract was administered (15mg of total phenols per Kg of animal weight), orally or intra-peritoneally from day 23 to the last day. Not only there was a statistically significant reduction in paw edema, but also the radiographic analysis of the animals’ paws showed that osteosclerosis of the articular surfaces and subchondral erosive areas of the joints of the hind paws were less evident in rats. In these, oral and intraperitoneal extract were administered. This suggests that there is an anti-inflammatory effect of the raspberry extract when it is continually administered.
Raspberries are classified as a berry and have a high content of phenolic compounds. Those compounds have been associated with health benefits due to their antioxidant and antiinflammatory effects. The main objective was to evaluate the anti-inflammatory effect of a raspberry extract in the animal model Arthritis Rheumatoid. It was prepared from an extract of an unknown raspberry variety from the south of Portugal. The extract was chemically analysed by spectrophotometric and chromatographic assays (HPLC-DAD-ED e HPLC-DAD-ESI-MS/MS). The antioxidant activity of the raspberry extract was evaluated by ORAC (Oxygen Radical Absorbance Capacity), HORAC (Hydroxyl Radical Antioxidant Capacity) and CAA (Cellular Antioxidant Activity) assays. It has obtained values of 1277 ± 38 μmol of Trolox equivalents per 100 g of fresh fruit, 529 ± 33 μmol of cafeic acid equivalents/ 100 g and 133 μmol of quercetin equivalents/ 100 g, respectively. For the acute inflammation study, in the carrageenaan induced-paw edema model, a dose of the raspberry extract (15mg of total phenols per Kg of animal weight) was administred, orally and intra-peritoneally. After the statistical analysis (ANOVA and Bonferroni’s test) of the paw’s volume increase, it was demonstrated that the oral extract administration did not inhibit the paw’s volume increase (P<0,001 vs Control) but the intra-peritoneal extract administration did inhibit the inflammatory effect (P <0,001 vs carrageenaan). The duration of the chronic inflammation assay was 35 days. It was an animal model of collagen-induced arthritis. The raspberry extract was administered (15mg of total phenols per Kg of animal weight), orally or intra-peritoneally from day 23 to the last day. Not only there was a statistically significant reduction in paw edema, but also the radiographic analysis of the animals’ paws showed that osteosclerosis of the articular surfaces and subchondral erosive areas of the joints of the hind paws were less evident in rats. In these, oral and intraperitoneal extract were administered. This suggests that there is an anti-inflammatory effect of the raspberry extract when it is continually administered.
Descrição
Tese de mestrado, Controlo de Qualidade e Toxicologia dos Alimentos, Universidade de Lisboa, Faculdade de Farmácia, 2011
Palavras-chave
Framboesa Inflamação Edema Artrite Reumatóide Teses de mestrado - 2011