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Infliximab microencapsulation: an innovative approach for intra-articular administration of biologics in the management of rheumatoid arthritis in vitro evaluation

2023-12Artigo científico Acesso aberto
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dc.contributor.authorLamela-Gómez, Iván
dc.contributor.authorGonçalves, Lídia
dc.contributor.authorAlmeida, António José
dc.contributor.authorLuzardo-Álvarez, Asteria
dc.date.accessioned2023-11-30T13:20:20Z
dc.date.available2023-11-30T13:20:20Z
dc.date.issued2023-12
dc.date.updated2023-11-22T19:44:07Z
dc.descriptionOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.pt_PT
dc.description.abstractMicroencapsulation of the therapeutical monoclonal antibody infliximab (INF) was investigated as an innovative approach to improve its stability and to achieve formulations with convenient features for intra-articular administration. Ultrasonic atomization (UA), a novel alternative to microencapsulate labile drugs, was compared with the conventional emulsion/evaporation method (Em/Ev) using biodegradable polymers, specifically Polyactive® 1000PEOT70PBT30 [poly(ethylene-oxide-terephthalate)/poly(butylene-terephthalate); PEOT-PBT] and its polymeric blends with poly-(D, L-lactide-co-glycolide) (PLGA) RG502 and RG503 (PEOT-PBT:PLGA; 65:35). Six different formulations of spherical core–shell microcapsules were successfully developed and characterized. The UA method achieved a significantly higher encapsulation efficiency (69.7–80.25%) than Em/Ev (17.3–23.0%). Mean particle size, strongly determined by the microencapsulation method and to a lesser extent by polymeric composition, ranged from 26.6 to 49.9 µm for UA and 1.5–2.1 µm for Em/Ev. All formulations demonstrated sustained INF release in vitro for up to 24 days, with release rates modulated by polymeric composition and microencapsulation technique. Both methods preserved INF biological activity, with microencapsulated INF showing higher efficacy than commercial formulations at comparable doses regarding bioactive tumor necrosis factor-alpha (TNF-α) neutralization according to WEHI-13VAR bioassay. Microparticles’ biocompatibility and extensive internalization by THP-1-derived macrophages was demonstrated. Furthermore, high in vitro anti-inflammatory activity was achieved after treatment of THP-1 cells with INF-loaded microcapsules, significatively reducing in vitro production of TNF-α and interleucine-6 (Il-6).pt_PT
dc.description.sponsorshipOpen Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. Iván Lamela-Gómez received funding from the “Axudas á etapa predoutoral da Xunta de Galicia, cofinanciadas polo programa operativo FSE Galicia 2014–2020” predoctoral grant program. Consellería de Cultura, Educación e ordenación universitaria, Xunta de Galicia, Spain. This research was partially funded by Consellería de Cultura, Educación e ordenación universitaria, Xunta de Galicia, Spain. Axudas para a consolidación e estructuración de unidades de investigación competitivas Modalidad A: Grupos de Referencia Competitiva (ED341C 2017/13). This research was also partially funded by the Fundação para a Ciência e a Tecnologia (FCT), Portugal (UID/DTP/04138/2019 and UIDB/04138/2020 to iMed.ULisboa), and principal investigator grants CEECIND/03143/2017 (L. M. Gonçalves).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLamela-Gómez I, Gonçalves LM, Almeida AJ, Luzardo-Álvarez A. Infliximab microencapsulation: an innovative approach for intra-articular administration of biologics in the management of rheumatoid arthritis—in vitro evaluation. Drug Deliv and Transl Res. 2023;13(12):3030–58. Disponível em: https://link.springer.com/10.1007/s13346-023-01372-1pt_PT
dc.identifier.doi10.1007/s13346-023-01372-1pt_PT
dc.identifier.slugcv-prod-3406045
dc.identifier.urihttp://hdl.handle.net/10451/61036
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationED341C 2017/13pt_PT
dc.relationResearch Institute for Medicines
dc.relationResearch Institute for Medicines
dc.relationNot Available
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s13346-023-01372-1pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectInfliximabpt_PT
dc.subjectMicroencapsulationpt_PT
dc.subjectRheumatoid arthritispt_PT
dc.subjectIntra-articularpt_PT
dc.subjectControlled releasept_PT
dc.subjectMonoclonal antibodiespt_PT
dc.titleInfliximab microencapsulation: an innovative approach for intra-articular administration of biologics in the management of rheumatoid arthritis in vitro evaluationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FDTP%2F04138%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F03143%2F2017%2FCP1476%2FCT0005/PT
oaire.citation.endPage3058pt_PT
oaire.citation.startPage3030pt_PT
oaire.citation.titleDrug Delivery and Translational Researchpt_PT
oaire.citation.volume13pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC IND 2017
person.familyNameLamela-Gómez
person.familyNameDiogo Gonçalves
person.familyNameLeitão das Neves Almeida
person.familyNameLuzardo Álvarez
person.givenNameIván
person.givenNameLídia Maria
person.givenNameAntónio José
person.givenNameAsteria
person.identifierI-6590-2012
person.identifier.ciencia-id7211-22BA-86AD
person.identifier.ciencia-id8312-F853-C47A
person.identifier.orcid0000-0002-1339-3101
person.identifier.orcid0000-0002-6799-2740
person.identifier.orcid0000-0002-7807-4726
person.identifier.orcid0000-0002-8842-0960
person.identifier.ridC-8396-2018
person.identifier.ridL-1783-2014
person.identifier.scopus-author-id57210283377
person.identifier.scopus-author-id57195052432
person.identifier.scopus-author-id6507933879
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid7211-22BA-86AD | Lídia Maria Diogo Gonçalves
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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