Logo do repositório
 
Publicação

Hsa_circ_0006571 promotes spinal metastasis through sponging microRNA-138 to regulate sirtuin 1 expression in lung adenocarcinoma

2020Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorWang, Hou-Lei
dc.contributor.authorWang, Hui-Ren
dc.contributor.authorLiang, Yun
dc.contributor.authorHu, An-Nan
dc.contributor.authorEnguita, Francisco J.
dc.contributor.authorZhou, Xiao-Gang
dc.contributor.authorDong, Jian
dc.date.accessioned2021-04-06T11:58:36Z
dc.date.available2021-04-06T11:58:36Z
dc.date.issued2020
dc.descriptionCopyright © 2009 - 2021 AME Publishing Company. All rights reserved. Open Access Statement:This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.description.abstractBackground: Circular RNAs (circRNAs) are known to participate in lung cancer. However, their role in spinal metastasis (SM) of lung adenocarcinoma remains elusive. In this study, we determined that hsa_circ_0006571 serves as a sponge for miR-138, which targets sirtuin 1 (Sirt1) in the development of SM. Methods: A human circRNA microarray was performed to compare SM and lung adenocarcinoma samples. The expression of hsa_circ_0006571 and miR-138 was determined using quantitative polymerase chain reaction (qPCR) in vitro and in vivo. Cell proliferation was performed by Cell Counting Kit-8 (CCK-8) and apoptosis was analyzed by Annexin V/PI staining. RNA-pulldown and RNA immunoprecipitation (RIP) were used to analyze the interaction between hsa_circ_0006571. Tumor metastasis was determined through a xenograft experiment in vivo. Results: Hsa_circ_0006571 was observed to be significantly upregulated in SM tissues through circRNA microarray and qPCR. We detected a lower expression of miR-138 in SM tissues compared with lung adenocarcinoma. Hsa_circ_0006571 silencing suppressed lung cancer cell proliferation and migration while promoting apoptosis. Hsa_circ_0006571 interacted with miR-138 to promote expression of Sirt1, leading to activation of epithelial-mesenchymal transition (EMT). Xenograft experiments showed that downregulation of hsa_circ_0006571 delayed the SM of lung adenocarcinoma cells via the miR-138-Sirt1 axis. Conclusions: Hsa_circ_0006571 promoted tumor cell migration and invasion via the miR-138/Sirt1 pathway. Our observations indicate that circRNAs are possible novel therapeutic targets for SM of lung adenocarcinoma.pt_PT
dc.description.sponsorshipThis work was supported by the National Natural Science Foundation of China (81572629, 81772855 and 81701370).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTransl Lung Cancer Res. 2020 Dec;9(6):2411-2427pt_PT
dc.identifier.doi10.21037/tlcr-20-1250pt_PT
dc.identifier.eissn2226-4477
dc.identifier.issn2218-6751
dc.identifier.urihttp://hdl.handle.net/10451/47248
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAME Publishing Companypt_PT
dc.relation.publisherversionhttps://tlcr.amegroups.com/pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectRNA circularpt_PT
dc.subjectLung adenocarcinomapt_PT
dc.subjectMetastasispt_PT
dc.subjectmiR-138-5ppt_PT
dc.subjectSpinept_PT
dc.titleHsa_circ_0006571 promotes spinal metastasis through sponging microRNA-138 to regulate sirtuin 1 expression in lung adenocarcinomapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2427pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage2411pt_PT
oaire.citation.titleTranslational Lung Cancer Researchpt_PT
oaire.citation.volume9pt_PT
person.familyNameEnguita
person.givenNameFrancisco J.
person.identifier173306
person.identifier.ciencia-idB215-8D49-3218
person.identifier.orcid0000-0002-8072-8557
person.identifier.ridA-2347-2009
person.identifier.scopus-author-id6602119231
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa101dbd4-6bf5-4546-882e-e2596eddb6b4
relation.isAuthorOfPublication.latestForDiscoverya101dbd4-6bf5-4546-882e-e2596eddb6b4

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
Hsa_0006571.pdf
Tamanho:
2.68 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

IMM - Artigos em Revistas Internacionais
FM - Artigos em Revistas Internacionais