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Publicação
Pesquisa de fitoplasmas das pomóideas e prunóideas em Portugal
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Autores
Resumo(s)
Phytoplasmas are unicellular organisms, prokaryotes, cell wall-less that cause
diseases in hundreds vegetable species.
This study is mainly about the prospection of “Candidatus” phytoplasma pyri,
“Candidatus” phytoplasma mali and “Candidatus” phytoplasma prunorum, etiologic agents of
PD, AP e ESFY, vectored by psyllids Cacopsylla pyri, Cacopsylla picta e Cacopsylla pruni,
respectively. These three phytoplasmas belong to AP or 16SrX group, considered
quarantine organisms (A2 list, EPPO).
In this study, two DNA extraction methods as well as two identification methods using
PCR were compared. The two extraction methods differ in lyses buffer which seems to be
crucial to obtain high quality DNA. The most used identification method until recently is
nested-PCR but as it is a time consuming and expensive method, it’s being replaced by realtime
PCR a quicker, more viable and less expensive one, as it was conf irmed in this study.
Plant and insect samples from pome and prunus fruit trees in Oeste area were
analysed.
With major importance lies the fact that, for the first time, we report the presence of
“Candidatus” phytoplasma pyri in insect and plant samples.
Descrição
Mestrado em Engenharia Agronómica - Protecção das Plantas - Instituto Superior de Agronomia
Palavras-chave
phytoplasmas 16SrX group psyllids nested-PCR real-time PCR Candidatus phytoplasma pyri
Contexto Educativo
Citação
Marques, Ana Isabel Ambrósio - Pesquisa de fitoplasmas das pomóideas e prunóideas em Portugal. Lisboa: ISA, 2011