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Effect of dietary Spirulina (Arthrospira platensis) on the intestinal function of post-weaned piglet: An approach combining proteomics, metabolomics and histological studies

2022-10Artigo científico Acesso restrito
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dc.contributor.authorMartins, Cátia Falcão
dc.contributor.authorRibeiro, D. M.
dc.contributor.authorMatzapetakis, Manolis
dc.contributor.authorPinho, Mário A.
dc.contributor.authorKuleš, Josipa
dc.contributor.authorHorvatić, Anita
dc.contributor.authorGuillemin, Nicolas
dc.contributor.authorEckersall, Peter David
dc.contributor.authorFreire, João
dc.contributor.authorAlmeida, André
dc.contributor.authorPrates, José A. M.
dc.date.accessioned2023-01-24T15:58:02Z
dc.date.available2023-01-24T15:58:02Z
dc.date.issued2022-10
dc.description.abstractThe effect of dietary Spirulina (Arthrospira platensis) and CAZyme supplementation was assessed on the gut of weaned piglets, using an integrated NMR-metabolomics approach combined with Tandem Mass Tag labelled proteomics. Thirty weaned male piglets were assigned to one of the three following diets (n = 10): cereal and soybean meal basal diet (Control), basal diet with 10% Spirulina inclusion (SP) and SP diet supplemented with 0.01% lysozyme (SP + L). The experiment lasted 4 weeks and, upon slaughter, small intestine samples were collected for histological, metabolomic and proteomic analysis. No significant differences were found for the histology and metabolomics analysis between the three experimental groups. Lactate, glutamate, glycine and myo-inositol were the most abundant metabolites. Proteomics results showed 1502 proteins identified in the intestine tissue. A total of 23, 78, 27 differentially abundant proteins were detected respectively for the SP vs. Control, SP + L vs. Control and SP + L vs. SP comparisons. The incorporation of Spirulina and supplementation of lysozyme in the piglet’s diets is associated to intestinal proteomic changes. These include increased protein synthesis and abundance of contractile apparatus proteins, related with increased nutrient availability, which has beneficial (increased glucose uptake) and detrimental (increased digesta viscosity) metabolic effects. Significance: The use of conventional feedstuffs becomes increasingly prohibitive due to its environmental toll. To increase the sustainability of the livestock sector, novel feedstuffs such as microalgae need to be considered. However, its recalcitrant cell wall has antinutritional effects that can inhibit high dietary inclusion levels. The supplementation with CAZymes is a possible solution to this issue. The small intestine is a central piece in monogastric digestion and of particular importance for the weaned piglet. Studying the effect of dietary Spirulina and CAZyme supplementation on its histomorphology, metabolome and proteome allows studying relevant physiological adaptations to these diets.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMartins, C. F., Ribeiro, D. M., Matzapetakis, M., Pinho, M.A. , Kuleš, J., Horvatić, A., Guillemin, N., Eckersall, P.D., Freire, J.P.B. , Almeida, A.M., Prates, J.A.M. Effect of dietary Spirulina (Arthrospira platensis) on the intestinal function of post-weaned piglet: An approach combining proteomics, metabolomics and histological studies. Journal of Proteomics, Volume 269, 2022, 104726pt_PT
dc.identifier.doihttps://doi.org/10.1016/j.jprot.2022.104726pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/27019
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation08/SI/3399/2015pt_PT
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relationLinking Landscape, Environment, Agriculture and Food
dc.relationInclusion of Laminaria and Ulva seaweeds as a feedstuff in piglet diets: effect on animal performance and physiology
dc.relation.publisherversionwww.elsevier.com/locate/jprotpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectspirulinapt_PT
dc.subjectpigletspt_PT
dc.subjectintestinept_PT
dc.subjectmetabolomicspt_PT
dc.subjectproteomicspt_PT
dc.titleEffect of dietary Spirulina (Arthrospira platensis) on the intestinal function of post-weaned piglet: An approach combining proteomics, metabolomics and histological studiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardTitleLinking Landscape, Environment, Agriculture and Food
oaire.awardTitleInclusion of Laminaria and Ulva seaweeds as a feedstuff in piglet diets: effect on animal performance and physiology
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCVT-NUT%2F5931%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04129%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F143992%2F2019/PT
oaire.citation.issueOctober 2022, 104726pt_PT
oaire.citation.titleJournal of Proteomicspt_PT
oaire.citation.volume269pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFalcão Martins
person.familyNameRibeiro Viera Santos
person.familyNameBengala Freire
person.familyNameMartinho de Almeida
person.givenNameCátia
person.givenNameDavid Miguel
person.givenNameJoão Pedro
person.givenNameAndré
person.identifier.ciencia-id5A14-15B0-2682
person.identifier.ciencia-id2714-8393-F2A3
person.identifier.ciencia-id871A-F6C8-60FE
person.identifier.ciencia-id1E14-92CC-B163
person.identifier.orcid0000-0002-1017-3269
person.identifier.orcid0000-0002-0990-7739
person.identifier.orcid0000-0002-1811-4378
person.identifier.orcid0000-0002-7810-3988
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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