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Illustrated keys and a DNA barcode reference library of the amphibians and terrestrial reptiles (Amphibia, Reptilia) of São Tomé and Príncipe (Gulf of Guinea, West Africa)
Publication . Ceríaco, Luis Miguel Pires; Marques, Mariana Pimentel; Sousa, Ana Carolina Andrade de; Veríssimo, Joana; Beja, Pedro; Ferreira, Sónia
The herpetofauna of São Tomé and Príncipe consists of nine species of amphibians, all
endemic, and 21 species of terrestrial reptiles, of which 17 are endemic. Our current knowl-
edge regarding its natural history, ecology, and distribution is limited. Here two important
tools are provided to support researchers, conservationists, and local authorities in the
identification of the country’s herpetofauna: an illustrated key to the herpetofauna of the
two islands and surroundings islets and a DNA barcode reference library. The keys allow
a rapid and unambiguous morphological identification of all occurring species. The DNA
barcodes for the entire herpetofauna of the country were produced from 79 specimens,
all of which are deposited in museum collections. The barcodes generated are available
in online repositories and can be used to provide unambiguous molecular identification
of most of the species. Future applications and use of these tools are briefly discussed
Hidden Secrets of Mangrove Swamp Rice Stored Seeds in Guinea-Bissau: Assessment of Fungal Communities and Implications for Food Security
Publication . Conde, Sofia; Barai, Amidu; Catarino, Sílvia; Costa, Gonçalo J.; Ferreira, Sónia; Tavares, Idília; Ferreira, Maria Rosa; Temudo, Marina Padrão; Monteiro, Filipa
Rice cultivation is one of the most important agro-economic activities in many countries,
and the correct seed storage between production cycles is essential for crop success. In Guinea-
Bissau, mangrove swamp rice (MSR) is a highly productive rice cropping system, thus providing
surplus for sale. Depending on storage conditions, rice grains may present moisture, insects, or
the development of fungi that might affect crop productivity. Considering different rice varieties
and storage conditions, samples were collected from 30 farmers within 13 villages across the main
MSR-producing regions. Stored rice was used to isolate and identify seed-borne fungi through DNA
barcoding, to test aflatoxin content, and to evaluate seed germination rates. Polyethylene bags (the
container), raised wooden platforms, and storage rooms (the structures) are the most used facilities.
Hermetic containers were recorded mainly in Oio. A total fungal richness of 18 genera was found, and
16 different species were identified. The most represented fungal genera are Aspergillus, Curvularia,
and Bipolaris. Despite the presence of aflatoxin-producing fungal samples, they did not present
concerning levels for human consumption, and the germination rate was not affected regardless of
storage structures. These results provide a baseline on fungi occurrence in stored MSR seeds from
traditional facilities in Guinea-Bissau.
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Entidade financiadora
Fundação para a Ciência e a Tecnologia
Programa de financiamento
CEEC IND 3ed
Número da atribuição
2020.03526.CEECIND/CP1601/CP1649/CT0007