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Research Project
Linking Landscape, Environment, Agriculture and Food
Funder
Authors
Publications
Produtos alternativos para o controlo de doenças das plantas
Publication . Resende, Mário Lúcio Vilela; Rodrigues, Fabrício Ávila; Botelho, Délia Magna dos Santos; Ribeiro Junior, Pedro Martins; Reichel, Tharyn; Guerra-Guimarães, Leonor
Os fosfitos e os silicatos, embora considerados
nutrientes minerais das plantas, podem também
funcionar como produtos alternativos no
controlo de doenças em diferentes culturas. Estes
minerais inorgânicos podem atuar diretamente
contra os microrganismos patogénicos e/ou
induzir as respostas de defesa das plantas
Modulation of the berry skin transcriptome of cv. tempranillo induced by water stress levels
Publication . Carvalho, Luisa C; Ramos, Miguel J. N.; Faísca-Silva, David; Marreiros, Pedro; Fernandes, João C.; Egipto, Ricardo; Lopes, Carlos M.; Amâncio, Sara
Climate change in the Mediterranean area is making summers warmer and dryer. Grapevine
(Vitis vinifera L.) is mostly important for wine production in Mediterranean countries, and the variety
Tempranillo is one of the most cultivated in Spain and Portugal. Drought decreases yield and
quality and causes important economic losses. As full irrigation has negative effects on quality and
water is scarce in this region, deficit irrigation is often applied. In this research, we studied the
effects of two deficit irrigation treatments, Sustained Deficit Irrigation (SDI) and Regulated Deficit
Irrigation (RDI), on the transcriptome of grape berries at full maturation, through RNAseq. The
expression of differentially regulated genes (DEGs) was also monitored through RT-qPCR along
berry development. Most transcripts were regulated by water stress, with a similar distribution of
up- and down-regulated transcripts within functional categories (FC). Primary metabolism was the
more severely affected FC under water stress, followed by signaling and transport. Almost all DEGs
monitored were significantly up-regulated by severe water stress at veraison. The modulation of an
auxin response repression factor, AUX22D, by water stress indicates a role of this gene in the response
to drought. Further, the expression of WRKY40, a TF that regulates anthocyanin biosynthesis, may be
responsible for changes in grape quality under severe water stress.
Assessment of the agronomic value of manure-based fertilizers
Publication . Prado, Joana; Fangueiro, David; Alvarenga, Paula; Ribeiro, Henrique
Producing manure-based fertilizers (MBFs) with specific nutrient ratios is a solution to
overpassing the imbalance of nitrogen and phosphorus in manures, and a way to recycle manure’s
nutrients, promoting sustainable agricultural practices. Several MBFs with different tailored N:P
ratios (0.5:1, 1:1 and 2:1) were produced to determine their agronomic value in a pot experiment
with oat (0.5:1 ratio: cattle manure with pig slurry (CaM+PiS), cattle manure with poultry manure
(CaM+PoM) and poultry manure with superphosphate (PoM+SP); 1:1 ratio: poultry manure with
cattle slurry (PoM+CaS) and poultry manure with pig slurry (PoM+PiS); 2:1 ratio: cattle slurry
with the liquid fraction of cattle slurry (CaS+CaS-LIQ), pig slurry with the liquid fraction of pig
slurry (PiS+PiS-LIQ) and poultry manure with urea (PoM+U)). The performance of these MBFs was
compared with conventional mineral fertilizers (MFs) in sandy soils (Haplic Arenosols) with different
nutrient requirements. Oat fertilized with PoM+SP (0.5:1) and PoM+PiS (1:1) led to yields similar
to those obtained with the use of MFs (6.3 and 7.2 mg DM, respectively). The MBFs PoM+SP and
PoM+PiS, as well as PiS+PiS-LIQ (2:1), were agronomically equivalent to the MFs. N uptake with
those MBFs was equivalent to that obtained with the MFs. Replacing MFs with MBFs in the basal
fertilization of oat was demonstrated to be a solution to turn agriculture more sustainable by recycling
nutrients efficiently
Proteomic signatures uncover the early key players on Vitis vinifera cv. “Regent - Plasmopara viticola crosstalk
Publication . Figueiredo, Joana; Santos, Rita B.; Cavaco, A.R.; Guerra-Guimarães, Leonor; Leclerq, C.C.; Sousa, L.; Silva, M.C.; Renaut, J.; Silva, M.S.; Figueiredo, A.
Assessing Spatio-Temporal Dynamics of Deep Percolation Using Crop Evapotranspiration Derived from Earth Observations through Google Earth Engine
Publication . Ferreira, Antónia; Rolim, João; Paredes, Paula; Cameira, Maria do Rosário
Excess irrigation may result in deep percolation and nitrate transport to groundwater.
Furthermore, under Mediterranean climate conditions, heavy winter rains often result in high deep
percolation, requiring the separate identification of the two sources of deep percolated water. An
integrated methodology was developed to estimate the spatio-temporal dynamics of deep percolation,
with the actual crop evapotranspiration (ETc act) being derived from satellite images data
and processed on the Google Earth Engine (GEE) platform. GEE allowed to extract time series of
vegetation indices derived from Sentinel-2 enabling to define the actual crop coefficient (Kc act) curves
based on the observed lengths of crop growth stages. The crop growth stage lengths were then
used to feed the soil water balance model ISAREG, and the standard Kc values were derived from
the literature; thus, allowing the estimation of irrigation water requirements and deep drainage
for independent Homogeneous Units of Analysis (HUA) at the Irrigation Scheme. The HUA are
defined according to crop, soil type, and irrigation system. The ISAREG model was previously
validated for diverse crops at plot level showing a good accuracy using soil water measurements and
farmers’ irrigation calendars. Results show that during the crop season, irrigation caused 11 3% of
the total deep percolation. When the hotspots associated with the irrigation events corresponded
to soils with low suitability for irrigation, the cultivated crop had no influence. However, maize
and spring vegetables stood out when the hotspots corresponded to soils with high suitability for
irrigation. On average, during the off-season period, deep percolation averaged 54 6% of the
annual precipitation. The spatial aggregation into the Irrigation Scheme scale provided a method for
earth-observation-based accounting of the irrigation water requirements, with interest for the water
user’s association manager, and at the same time for the detection of water losses by deep percolation
and of hotspots within the irrigation scheme
Organizational Units
Description
Keywords
Contributors
Funders
Funding agency
Fundação para a Ciência e a Tecnologia
Funding programme
6817 - DCRRNI ID
Funding Award Number
UID/AGR/04129/2019