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Effect of moderate hydrostatic pressures on the enzymatic activity and bioactive composition of pineapple by-products
Publication . Santos, Diana I.; Pinto, Carlos A.; Corrêa-Filho, Luiz C.; Saraiva, Jorge A.; Vicente, António A.; Moldão, Margarida
The application of abiotic stresses by moderate hydrostatic pressures (MHP) is still
underdeveloped. Abiotic stresses allow activating the enzymatic complexes inducing
the synthesis of de novo bioactive compounds. Pineapple by-products are rich in bromelain
and bioactive compounds that can be enhanced through abiotic stresses. The
aim of this study was to evaluate the effect of MHP on the enzymatic activity of
pineapple by-products. Pineapple by-products were submitted to MHP (50–400 MPa
between 1 and 15 min) according to a central composite factorial design matrix. Samples
were stored at 5 ± 1 C for 24 hr, to allow enzymatic activity to occur. Enzymatic
and antioxidant activities and total phenolic compounds (TPC) were quantified. MHP
promoted a 262% increase in the phenylalanine ammonia-lyase activity and 36%
increase in TPC, in shell samples. In core the activity of bromelain increased 350%.
These results pinpoint the potential to increase the value of pineapple by-products
by enhancing the amounts of bioactive compounds through MHP application
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Fundação para a Ciência e a Tecnologia
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POR_CENTRO
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71386