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Projeto de investigação
Mathematical an Computational Modeling of Human Physiology
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Publicações
The ubiquity nature of acetylcholine
Publication . Saldanha, Carlota; Herdade, Ana S Silva
Application of a nitric oxide sensor in biomedicine
Publication . Saldanha, Carlota; Almeida, José Pedro Lopes de; Herdade, Ana Santos Silva
In the present study, we describe the biochemical properties and effects of nitric oxide (NO) in intact and dysfunctional arterial and venous endothelium. Application of the NO electrochemical sensor in vivo and in vitro in erythrocytes of healthy subjects and patients with vascular disease are reviewed. The electrochemical NO sensor device applied to human umbilical venous endothelial cells (HUVECs) and the description of others NO types of sensors are also mentioned.
Hydrodynamics of a free-flowing leukocyte toward the endothelial wall
Publication . Herdade, Ana Santos Silva; Sequeira, Adélia; Calado, Ângelo; Saldanha, Carlota; Kafi, Oualid
Leukocyte recruitment is an essential stage of the inflammatory response and although the molecular mechanisms of this process are relatively well known, the influence of the hydrodynamic effects that govern the inflammatory response are still under study. In this paper we made use of the images and experimental parameters obtained by intravital microscopy in an in vivo animal model of inflammation to track the leukocytes trajectories and measure their velocities and diameters. Using a recent validated mathematical model describing the coupled deformation-flow of an individual leukocyte in a microchannel, numerical simulations of an individual and of two leukocytes under flow were performed. The results showed that velocity plays an important role in the motion, deformation and attraction of the cells during an inflammatory response. In fact, for higher inlet velocities the cell movement along the endothelial wall is accelerated and the attraction forces break faster. These results highlight the role of the mechanical properties of the blood, namely the ones influenced by the velocity field, in the case of inflammation.
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Entidade financiadora
Fundação para a Ciência e a Tecnologia
Programa de financiamento
3599-PPCDT
Número da atribuição
EXCL/MAT-NAN/0114/2012