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Caffeine intake exerts dual genome-wide effects on hippocampal metabolism and learning-dependent transcription
Publication . Paiva, Isabel; Cellai, Lucrezia; Meriaux, Céline; Poncelet, Lauranne; Nebie, Ouada; Saliou, Jean-Michel; Lacoste, Anne-Sophie; Papegaey, Anthony; Drobecq, Hervé; Le Gras, Stéphanie; Schneider, Marion; Malik, Enas M.; Müller, Christa E.; Faivre, Emilie; Carvalho, Kevin; Gomez-Murcia, Victoria; Vieau, Didier; Thiroux, Bryan; Eddarkaoui, Sabiha; Lebouvier, Thibaud; Schueller, Estelle; Tzeplaeff, Laura; Grgurina, Iris; Seguin, Jonathan; Stauber, Jonathan; Lopes, Luisa V.; Buée, Luc; Buée-Scherrer, Valérie; Cunha, Rodrigo A.; Ait-Belkacem, Rima; Sergeant, Nicolas; Annicotte, Jean-Sébastien; Boutillier, Anne-Laurence; Blum, David
Caffeine is the most widely consumed psychoactive substance in the world. Strikingly, the molecular pathways engaged by its regular consumption remain unclear. We herein addressed the mechanisms associated with habitual (chronic) caffeine consumption in the mouse hippocampus using untargeted orthogonal omics techniques. Our results revealed that chronic caffeine exerts concerted pleiotropic effects in the hippocampus at the epigenomic, proteomic, and metabolomic levels. Caffeine lowered metabolism-related processes (e.g., at the level of metabolomics and gene expression) in bulk tissue, while it induced neuron-specific epigenetic changes at synaptic transmission/plasticity-related genes and increased experience-driven transcriptional activity. Altogether, these findings suggest that regular caffeine intake improves the signal-to-noise ratio during information encoding, in part through fine-tuning of metabolic genes, while boosting the salience of information processing during learning in neuronal circuits.
Transection of the superior sagittal sinus enables bilateral access to the rodent midline brain structures
Publication . Dias, Marcelo; Marques-Morgado, Inês; Coelho, Joana E; Ruivo, Pedro; Lopes, Luisa V.; Remondes, Miguel
Stereotaxic access to brain areas underneath the superior sagittal sinus (SSS) is notoriously challenging. As a major drainage vessel, covering the whole extension of the sagittal fissure, the SSS impedes direct bilateral access to underlying regions for recording and stimulation probes, drug-delivery cannulas, and injection devices. We now describe a new method for transection and retraction of the SSS in rats, that allows the accurate placement of microinjection devices, or chronic electrode probes, while avoiding hemorrhage and the ensuing deleterious consequences for local structures, animal health, and behavior. To demonstrate the feasibility of this approach we evaluated its consequences acutely during surgery, and thereafter during surgical survival, recovery, behavioral testing, as well as postmortem analysis of histologic impact in the related brain structures of male rats. This method provides a new approach enabling direct access for manipulation and recording of activity in brain areas previously obstructed by the SSS.
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Fundação para a Ciência e a Tecnologia
Programa de financiamento
CEEC IND 2017
Número da atribuição
CEECIND/01497/2017/CP1396/CT0004