PROPAGATION OF PLANTAIN, SUGARCANE AND PINEAPPLE PLANTLETS IN TEMPORARY IMMERSION BIOREACTORS TIB: ACQUISITION OF RESPECTIVELY C3, C4 OR CAM PHOTOSYNTHESIS AND ANTIOXIDANT COMPETENCE

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Physiological characteristics as analyzed by hormone profile, metabolic pathways and expression of specific induced genes of C3, C4 and CAM tropical crops propagated by Temporary Immersion Bioreactors (TIB)

Publication . Abreu, Carlos Eduardo Aragón; Amâncio, Sara Barros Queiróz; Carvalho, Luísa Cristina Ferreira

Physiology and biochemistry of in vitro plants can be complex and different when compared with conventional and well known plant ex vitro behavior. Metabolic reprogramming events that occur in a number of in vitro propagated plant species give rise to low ex vitro yields as a handicap for commercial application. Temporary Immersion Bioreactors (TIB) are a method to obtain plantlet morphology and physiology much alike that of in vivo grown plants. Three tropical crops were selected for their economic importance, but also because of their different photosynthetic characteristics, to compare C4 (sugarcane) and C3-CAM (pineapple) facultative photosynthetic pathways to the more common C3-type (plantain) photosynthesis. Experiments performed using plantain and sugarcane allowed to integrate the results obtained when monitoring the oxidative stress response in C3 and C4 metabolism. Pineapple plants propagated in TIB and evaluated during acclimatization under C3 and CAM inducing conditions were used to describe the facultative C3-CAM carbon metabolism and the influence of the environmental conditions on the switch from C3 to CAM. Studies based on the modulation of in vitro conditions which reproduce abiotic stress conditions can be used for understanding the influence of upcoming climate changes on the physiology of different species

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Entidade financiadora

Fundação para a Ciência e a Tecnologia

Programa de financiamento

FARH

Número da atribuição

SFRH/BD/43181/2008