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- Frontiers in fluorescence microscopyPublication . Rino, José; Braga, José; Henriques, Ricardo; Carmo-Fonseca, MariaHow we see organisms and cells depends on the tools at our disposal. For over 150 years, biologists were forced to rely on fixed, dehydrated and stained specimens in order to guess how the living cells could function. It all changed abruptly during the last two decades when the rapid development of novel imaging techniques revolutionized the way scientists look at the structures of life alive.