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- Problemas de conservação da pintura de cavalete : o acervo da Faculdade de Belas-Artes da Universidade de Lisboa (FBAUL)Publication . Freitas, Nelson Manuel Seixas da Silva dos Santos; Bailão, Ana Maria dos Santos; Pais, João Miguel Pereira CorreiaToday, the painting collection of the Faculty of Fine Arts of the University of Lisbon (FBAUL) has a vast and wide-ranging heritage, whose collections have been permanently enriched. The collection includes numerous paintings, divided into three rooms. This dissertation focuses on room 3.44, which holds FBAUL's Contemporary Painting Collection. In order to understand the collection's history and formation, the technical and material characteristics of the works and the agents of deterioration were analysed. Due to the fact that they had not been studied and inventoried, inventory records and a diagnosis of the state of conservation were created, in accordance with the painting inventory standards, in order to update knowledge of the FBAUL collection. We analysed 390 paintings produced after 1960, a group of paintings made by students of the Faculty during their teaching time, at the end of their course and as offers to the Faculty. We assessed the conditions of the room where the works are stored and checked the conditions of the room by measuring the intensity and type of lighting, monitoring humidity, temperature and pest control. The objectives of the research were: to inventory, characterize and quantify the constituent materials and techniques, the causes of deterioration and the degradation factors that led to the damage to the paintings. At the same time, a system for tracking the state of conservation in colour was defined for quick identification and essential for defining intervention priorities. During the process, due to the poor state of conservation of numerous works, preventative measures were taken and proposals put forward to improve the conditions of FBAUL's Contemporary Painting Collection.
- Epigenetic changes in equine myometrium at different stages of endometrosisPublication . Silva, Beatriz de Oliveira Celeiro e; Szóstek-Mioduchowska, Anna; Dias, Graça Maria Leitão FerreiraA reduction in the contractile activity of the myometrium can result in insufficient cleaning of the uterine lumen and the persistence of endometritis, leading to endometrosis. Studies in humans and other species point out to an important role for epigenetic mechanisms in gene expression regulation in the myometrium. However, in mares, there is a lack of information regarding the expression of enzymes responsible for DNA methylation (DNMTs) and demethylation (TETs) and the importance of these mechanisms. The main objective of this work was to determine the mRNA expression of these enzymes and explore the potential role of epigenetic mechanisms in the expression of genes encoding oxytocin (OXTR) and PGF2α (PTGFR) receptors in the myometrium of mares with endometrosis. Myometria were used in the midluteal (n=23) and follicular (n=20) phases of the estrous cycle. The material was grouped according to the corresponding endometrial classification (Kenney and Doig, 1986): category I (without pathological changes), IIA (mild endometrial fibrosis and/or inflammation), IIB (moderate endometrial fibrosis) and III (extensive endometrial fibrosis). Using qPCR method, the expression of DNMTs (DNMT1, DNMT3A, DNMT3B), TETs (TET1, TET2, TET3) and the expression levels of OXTR and PTGFR in the myometrium were determined. Using the bisulfite DNA pyrosequencing method, quantitative analysis (%) of DNA methylation was performed at specific sites (CpG islands) of two regions (exon 1 and 2) of OXTR gene. PTGFR did not have CpG islands and, thus, could not be analyzed. DNMT1 mRNA expression increased in myometrium in category IIA compared to category I in the follicular phase of the estrous cycle (P<0.05). The expression of TET2 mRNA was upregulated in the myometrium in categories I and IIB in the midluteal phase, compared to the follicular phase of the estrous cycle, also showing a positive regulation in category IIB compared to category I, in both phases (P <0.05). TET3 mRNA expression was increased in the myometrium in category IIA compared with category I in the follicular phase and was upregulated in category IIB in the follicular phase when compared with the midluteal phase of the estrous cycle (P<0.05). On the other hand, expression levels of OXTR and PTGFR and the global methylation (%) of OXTR in CpG islands did not show any significant differences between categories. However, an increase in DNA methylation was documented at position 6 of exon 2 of OXTR. The results suggest the potential regulatory role of epigenetic mechanisms in gene expression in the myometrium, related to changes in uterine contraction in mares with endometrosis
- O acontecimento em pinturaPublication . Antonio, Eduardo Ceron Lollato; Jacinto, JoãoThe objective of the present work project is to provide an intimate view of all the issues surrounding painting and its practice. Through personal accounts about the motivation for doing painting, the techniques used, the selection and research of references, and the act of painting itself, it seeks to enhance the understanding and thinking that underlies the creative process. The entire text was written in a continuous manner, without the existence of a predetermined index, with the intention of presenting a writing that resembles painting not only in terms of thought but also in its form. In other words, the writing occurred simultaneously with the act of painting. Therefore, the structure of the chapter was organized based on Herberto Helder’s short story O Quarto (The Room). Each one of them represents a room in the house described in the narrative of the story, only the first of them is about the choice of the place from which to build it. Finally, it was decided to present written fragments from a physical journal kept in the studio during the development of this work project and an appendix with drawings from a notebook created during the same period, almost like a journal of images