Browsing by Author "Sepodes, Bruno"
Now showing 1 - 10 of 26
Results Per Page
Sort Options
- Anti-inflammatory activity of naringin and the biosynthesised naringenin by naringinase immobilized in microstructured materials in a model of DSS-induced colitis in micePublication . Amaro, Maria Ines; Rocha, Joao; Vila-Real, Helder; Eduardo-Figueira, Maria; Mota-Filipe, Helder; Sepodes, Bruno; Ribeiro, Maria H.A great number of antioxidants are naturally present in citrus juice, being responsible for their potentially protective action against inflammation. Naringin, a bitter compound, in citrus, is hydrolysed by naringinase into tasteless naringenin and to rhamnose and glucose. The aim of this study was to evaluate the anti-inflammatory activity of naringin and naringenin in an acute model of induced colitis in mice. Microstructured particles were used for naringinase immobilization. The influence of naringin and naringinase concentration on the bioconversion was evaluated through a central composite design. Colitis was induced by feeding mice 7% dextran sodium sulphate (DSS) dissolved in drinking water for 5 days. Intestine dry/wet weight ratio (indicator of intestine edema), nitrates/nitrites (indicators of inflammatory process), and tissue malondialdehyde levels (indicator of lipid peroxidation) were evaluated. Our results indicate that treatment with both naringin and naringenin significantly reduced the formation of intestine edema, suggesting an anti-inflammatory activity in this model of colitis in mice. (C) 2009 Elsevier Ltd. All rights reserved.. - Fundacao para a Ciencia e Tecnologia, Portugal [SFRH/BD/30716/2006]. - Helder Vila Real was supported by Fundacao para a Ciencia e Tecnologia, Portugal. Grant No. SFRH/BD/30716/2006.
- Anti-inflammatory effect of lycopene on carrageenan-induced paw oedema and hepatic ischaemia-reperfusion in the ratPublication . Bignotto, Leticia; Rocha, Joao; Sepodes, Bruno; Eduardo-Figueira, Maria; Pinto, Rui; Chaud, Marco; de Carvalho, Joao; Moreno, Heitor,; Mota-Filipe, HelderThe regular intake of tomatoes or its products has been associated with a reduced risk of chronic diseases and these effects have been mainly attributed to lycopene. Here, we evaluated the anti-inflammatory properties of lycopene and its protective effects on organ injury in two experimental models of inflammation. In order to study the effects of lycopene in local inflammation, a carrageenan-induced paw oedema model in rats was performed. Lycopene was administered as an acute (1, 10, 25 or 50 mg/kg, intraperitoneally, 15 min before carrageenan injection) and chronic treatment (25 or 50 mg/kg per d, 14 d). Inflammation was assessed by the measurement of paw volume increase after 6 h. Lycopene significantly inhibited paw oedema formation at two doses (25 and 50 mg/kg) in both acute and repeated administration. The effect of lycopene on liver inflammation was evaluated in a liver ischaemia-reperfusion (I/R) model. Rats were subjected to 45 min of ischaemia of three-quarters of the liver followed by 2h of reperfusion. In this model, lycopene was administered daily at two doses (25 and 50mg/kg) during the 14d that preceded the experiments. Repeated administration of lycopene reduced liver injury induced by I/R, as demonstrated by the reduction of the increase in liver injury markers (aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase and gamma-glutamyl transferase) and attenuation of liver tissue lipoperoxidation was evidenced by a decrease in malondialdehyde production. The present results show that lycopene exhibited local anti-inflammatory activity and also attenuated liver injury induced by I/R. We speculate that lycopene administration might be useful in the pharmacological modulation of inflammatory events.
- ANTI-INFLAMMATORY EFFECT OF ROSMARINIC ACID ON A MODEL OF CARRAGEENAN-INDUCED PAW EDEMAPublication . Rocha, Joao; Sepodes, Bruno; Eduardo-Figueira, Maria; Bronze, Maria Rosario; Lima, Beatriz Silva; Mota-Filipe, Helder
- Characterisation of cystathionine gamma-lyase/hydrogen sulphide pathway in ischaemia/reperfusion injury of the mouse kidneyPublication . Tripatara, Pinpat; Patel, Nimesh S. A.; Brancaleone, Vincenzo; Renshaw, Derek; Rocha, Joao; Sepodes, Bruno; Mota-Filipe, Helder; Perretti, Mauro; Thiemermann, ChristophThe generation of endogenous hydrogen sulphide may either-limit or or contribute to the degree of tissue injury caused by ischaemia/reperfusion injury. Here, we have attempted to characterise the endogenous hydrogen sulphide synthesis pathway and the effects of sodium hydrosulphide, a hydrogen sulphide donor, in a mouse model of renal ischaemia/reperfusion injury. Anaesthetised male C57/b mice weighing 20-25 g were divided into two groups; (i) 'Ischaemia/Reperfusion Injury', in which mice were subjected to bilateral renal ischaemia performed by clamping the renal pedicles for 30 min followed by reperfusion for 24 h, (ii) 'Sham', in which mice were subjected to the same surgical procedures as above, except for renal ischaemia/reperfusion. Western blot analysis of the kidney taken at the end of the experiment demonstrated that cystathionine gamma-lyase, the enzyme responsible for generating hydrogen sulphide in the cardiovascular system, is expressed in the normal kidney and is significantly increased after ischaemia/reperfusion injury. Ischaemia/reperfusion injury significantly increased the rate of hydrogen sulphide production in kidney homogenates and increased the plasma concentration of hydrogen sulphide. In addition, we have shown that administration of the hydrogen sulphide donor sodium hydrosulphide (100 mu mol/kg) 30 min prior to ischaemia and 6 h into reperfusion significantly attenuated ischaemia/reperfusion injury-induced renal dysfunction indicated by serum creatinine and Urea. These findings suggest that hydrogen sulphide protects the kidney against ischaemia/reperfusion injury and that the increase in expression of the enzyme cystathionine gamma-lyase during ischaemia/reperfusion injury may be one of many endogenous mechanisms to limit renal ischaemia/reperfusion injury. (C) 2009 Elsevier B.V. All rights reserved.
- Effect of naringin enzymatic hydrolysis towards naringenin on the anti-inflammatory activity of both compoundsPublication . Ribeiro, Isabel A.; Rocha, Joao; Sepodes, Bruno; Mota-Filipe, Helder; Ribeiro, Maria H.The aim of this work was the hydrolysis of naringin towards naringenin with immobilized naringinase and the evaluation of their anti-inflammatory activity. An acute local inflammation model (rat paw edema induced by X-carrageenan) was chosen to evaluate the contribution of antioxidant properties to a possible anti-inflammatory effect. Grapefruit juice was processed with naringinase immobilized in k-carrageenan (2%) beads (approximate to 3 mm). A 95% naringin conversion in grapefruit juice was obtained with immobilized naringinase (1000 mg L-1), with an activity of 19.5 mg mL(-1) min(-1) and the formation of 215 mg L-1 of naringenin. Ascorbic acid and indometacine were used as positive anti-inflammatory controls. All results were analysed using ANOVA with Dunnett's Post-test. The results show that rats (n = 9) pre-treated with a solution of naringin, rats (n = 9) pre-treated with a solution of naringenin (in concentrations equal as in grapefruit juice) and rats (n = 9) treated with a solution of naringenin and naringin, revealed a significant reduction on edema formation, 6h after X-carrageenan injection. Naringenin demonstrated a high in vivo anti-inflammatory activity, only 8% of paw edema (p0.001) was observed in rats pre-treated with a solution of naringenin. Comparability studies, in rats administered orally with grapefruit juice (before and after processing), showed that enzymatic processing did not affect the anti-inflammatory properties of the juice. (C) 2007 Elsevier B.V. All rights reserved.
- Exercise training is associated with improved levels of C-reactive protein and adiponectin in ZDF (type 2) diabetic ratsPublication . de Lemos, Edite Teixeira; Reis, Flavio; Baptista, Sofia; Pinto, Rui; Sepodes, Bruno; Vala, Helena; Rocha-Pereira, Petronila; Silva, Alice Santos; Teixeira, FredericoBackground: Chronic tow-grade systemic inflammation is a feature of such chronic diseases as cardiovascular disease and type 2 diabetes (T2D). There is evidence that regular exercise is effective as a treatment in these situations. This study intended to assess the levels of two inflammatory mediators, C-creative protein (CRP) and adiponectin, in Zucker Diabetic Fatty (ZDF, fa/fa) rats, an experimental model of T2D, and to determine whether exercise-induced changes in insulin resistance could be explained by modifications in these inflammation markers. Material/Methods: Male ZDF (Gmi fa/fa) rats and their littermates (Gmi +/+), aged 8 weeks, were randomly assigned in two groups: an exercise trained and a sedentary one. Swimming was conducted 1 h/day 3 days/week for 12 weeks. The rats were sacrificed 48 h after the last rotund of exercise. Rats had their body weight, insulin, adiponectin, CRP, as well as glucose, total cholesterol, triglycerides, MDA, and SOD measured and HOMA-IR calculated before and after the 12-week swimming training. Results: In the ZDF (fa/fa) rats underwent swimming exercise, all the metabolic abnormalities were totally or partially prevented (p 0.001), namely the hyperglycemic, hyperinsulinemic, and dyslipidemic pattern observed in their sedentary counterparts. Furthermore, even without body weight change, a plasma adiponectin increase (28.0%) and a CRP decrease (12.7%) were also observed. Conclusion: A 12-week thrice-weekly swimming training was associated with improved measures of chronic inflammation markers as measured by adiponectin and CRP. Moreover, improvements in insulin sensitivity resulting from swimming exercise appeared to be related to changes in these inflammatory mediators.
- Glycogen synthase kinase-3b inhibitor, TDZD-8, attenuates the liver injury caused by ischemia-reperfusion in the ratPublication . Sepodes, Bruno; Abdelrahman, Maha; Pinto, Rui; Figueira, Maria-Eduardo; Mota-Filipe, Helder; Thiemermann, Christoph
- Hypocholesterolemic effect of green tea and antioxidant effect ofepigallocatechin gallate (EGCG) from green teaPublication . Reto, Marcia; Almeida, Cristina M.M.; Barroso, Isabel; Sepodes, Bruno; Pinto, Rui; Mota-Filipe, Helder; Figueira, Maria-Eduardo
- Inhibition of endogenous hydrogen sulphide formation protects the liver from scald burn injury in the ratPublication . Sepodes, Bruno; Rocha, Joao; Marques, Patricia; Pinto, Rui; Thiemermann, Christoph; Mota-Filipe, Helder
- Insulin and TDZD-8, a glycogen synthase kinase-3 beta inhibitor, reduce the liver injury caused by ischemia-reperfusion in the ratPublication . Sepodes, Bruno; Rocha, Joao; Pinto, Rui; Thiemermann, Christoph; Mota-Filipe, Helder
- «
- 1 (current)
- 2
- 3
- »