Browsing by Author "Pimentel, M"
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- Chromosomal class C beta-lactamase in Enterobacter cloacae strain in a Portuguese hospitalPublication . Conceição, T; Faria, N; Lito, LM; Cristino, JMelo; Salgado, MJ; Pimentel, M; Duarte, A
- Expression of mycobacteriophage Ms6 lysis genes is driven by two sigma(70)-like promoters and is dependent on a transcription termination signal present in the leader RNAPublication . Garcia, M; Pimentel, M; Moniz-Pereira, JA mycobacteriophage Ms6 strong promoter region (P-tys) was isolated by using transcriptional fusions with the lacZ reporter gene. Two tandem or sigma(70)-like promoter sequences (P1 and P2) were found in this region. DNA sequencing of the promoter downstr
- High-level expression of IMP-5 carbapenemase owing to point mutation in the -35 promoter region of class 1 integron among Pseudomonas aeruginosa clinical isolatesPublication . Brizio, A; Conceicao, T; Pimentel, M; Da Silva, G; Duarte, AEight strains of Pseudomonas aeruginosa producing IMP-5 carbapenemases were collected from three Portuguese hospitals. All isolates were epidemiologically unrelated. The bla(IMP-5) gene was inserted into a class I integron previously reported in Acinetobacter baumannii 65FFC. Expression of the the blaIMP-5 gene in P aeruginosa has been shown to be driven by the P, promoter [TTGATA] in which the cytosine was replaced by thymine, which caused an increase in transcription of blaIMP-5 that was confirmed by site-directed mutagenesis. The minimum inhibitory concentrations (MICs) of imipenem for Escherichia coli pGMLA-1 (the recombinant front A. baumannii 65FFC) and E. coli pATG-2 (the recombinant from P. aeruginosa isolates) were 0.5 mg/L and 32 mg/L, respectively. This study reports the spread of a class 1 integron In76 with a new point mutation in the P-1 promoter sequence leading to overproduction of INIP-5. (c) 2005 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
- New chromosomal AmpC beta-lactamase in Enterobacter cloacaePublication . Conceicao, T; Faria, N; Pimentel, M; Soveral, G; Duarte, A; Lito, LM; Cristino, JM; Salgado, MJ
- New Chromosomal AmpC ß-lactamase in Enterobacter cloacaePublication . Conceição, T; Faria, N; Lito, LM; Cristino, JMelo; Salgado, MJ; Pimentel, M; Soveral, G; Duarte, A
- Transduction of imipenem resistance by bacteriophages carried by Pseudomonas aeruginosa clinical isolatesPublication . Brizio, A; Silva, GDa; Duarte, A; Pimentel, M