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Browsing by Author "Domingos, Ana"

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  • Artemisinin-dipeptidyl vinyl sulfone hybrid molecules
    Publication . Capela, Rita; Oliveira, Rudi; Goncalves, Lidia M.; Domingos, Ana; Gut, Jiri; Rosenthal, Philip J.; Lopes, Francisca; Moreira, Rui
    A series of artemisinin-vinyl sulfone hybrid molecules with the potential to act in the parasite food vacuole via endoperoxide activation and falcipain inhibition was synthesized and screened for antiplasmodial activity and falcipain-2 inhibition. All conjugates were active against the Plasmodium falciparum W2 strain in the low nanomolar range and those containing the Leu-hPhe core inhibited falcipain-2 in low micromolar range. (C) 2009 Elsevier Ltd. All rights reserved.. - FCT (Portugal) [SFRH/BD/30418/2006]; National Institutes of Health. - This work was supported by FCT (Portugal) and the National Institutes of Health; R. C. acknowledges FCT for the PhD grant SFRH/BD/30418/2006. PJR is a Doris Duke Charitable Foundation Distinguished Clinical Scientist.
    2009Journal article Metadata only Show more
  • Plasmodium chabaudi
    Publication . Caldeira, Rubina L.; Goncalves, Lidia M. D.; Martins, Tiago M.; Silveira, Henrique; Novo, Carlos; do Rosario, Virgilio; Domingos, Ana
    Plasmodium cysteine proteases have been shown to be immunogenic and are being used as malaria potential serodiagnostic markers and vaccine targets. Genes encoding two Plasmodium chabaudi cysteine proteases chabaupain-1 (CP-1) and chabaupain-2 (CP-2) were identified and further expressed in Escherichia coli. Solubilisation of recombinant CP-1 and CP-2 was achieved by decreasing the temperature of induction. Anopheles gambiae tissues infected with Plasmodium were analyzed by Western blotting using the anti-CP-1 antibody showing that CP-1 is only present in the A. gambiae midguts being absent from other infected mosquito biological material. Anti-CP-1 anti-serum recognized a 30 kDa band in P. chabaudi, Plasmodium berghei and Plasmodium yoelii lysates but does not recognize the recombinant CP-2 extracts suggesting high antibody specificity. (c) 2009 Elsevier Inc. All rights reserved.. - Portuguese Foundation for Science and Technology (FCT) [POCTI/SAU-ESP/57696/2004]. - This research was funded by the Portuguese Foundation for Science and Technology (FCT) under the project POCTI/SAU-ESP/57696/2004.
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