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Browsing by Author "Anand, Paras K."

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  • Exosomal Hsp70 induces a pro-inflammatory response to foreign particles including mycobacteria
    Publication . Anand, Paras K.; Anand, Ellis; Bleck, Christopher K. E.; Anes, Elsa; Griffiths, Gareth
    Background: Exosomes are endosome-derived vesicles that are released when multi-vesicular bodies (MVBs) fuse with the plasma membrane. Exosomes released from mycobacteria-infected cells have recently been shown to be pro-inflammatory. A prominent host molecule that is found within these exosomes is Hsp70, a member of the heat-shock family of proteins. Methodology/Principal Findings: We first characterized the exosomes purified from control and mycobacteria-infected cells. We found that relative to uninfected cells, macrophages infected with M. smegmatis and M. avium release more exosomes and the exosomes they released had more Hsp70 on their surface. Both exosomes and exogenous Hsp70 treatment of macrophages led to NF-kB activation and TNFa release in uninfected macrophages; Hsp70 levels were elevated in mycobacteria-infected cells. Macrophage treatment with Hsp70 also led to increase in the phagocytosis and maturation of latex-bead phagosomes. Finally, Hsp70 pre-incubation of M. smegmatis- and M. avium-infected cells led to increased phago-lysosome fusion, as well as more killing of mycobacteria within macrophages. Conclusions/Significance: Our results fit into an emerging concept whereby exosomes-containing Hsp70 are effective inducers of inflammation, also in response to mycobacterial infection.
    2010-04Journal article Open access Show more
  • Lipids regulate P2X7-receptor-dependent actin assembly by phagosomes via ADP translocation and ATP synthesis in the phagosome lumen
    Publication . Kuehnel, Mark. P.; Rybin, Vladimir; Anand, Paras K.; Anes, Elsa; Griffiths, Gareth
    Latex bead phagosomes isolated from J774 macrophages polymerize actin. We show here that five lipids phosphatidylinositol-4-phosphate, phosphatidylinositol-(4,5)bisphosphate, sphingosine-1-phosphate (S1P), ceramide-1-phosphate and phosphatidic acid - stim. - Fundacao para a Ciencia e a tecnologia (FCT). - We would like to thank Sabrina Marion and Simi Antony for their technical support. We greatly appreciate the suggestions by Jens Reich and Thomas Dandekar. Thanks also to Christopher Bleck for preparing Fig. 4 and to Luis Mayorga, Sabrina Marion and Maxim
    2009Journal article Metadata only Show more